问答题 It is well known that biological changes at the molecular level have morphogenetic consequences, consequences affecting the formation and differentiation of tissues and organs. It is superfluous to point out that gene mutations and disturbances of the biosynthetic processes in the embryo may result in abnormalities in the morphology (structure) of an organism. However, whereas much is known about causes and consequences at the molecular level, and in spite of an enormous accumulation of chemical and morphological data on embryos of various kinds, {{U}}our understanding of how genes control morphogenesis is still far from complete. Perhaps one reason for this is that molecular biologists and morphologists speak different languages.{{/U}} Whereas the former speak about messenger-RNA and conformational changes of protein molecules, the latter speak of ectoderms, hypoblasts, and neural crests. {{U}}One solution to this predicament is to try to find some phenomena relevant to morphogenesis which both the molecular biologist and the morphologist can understand and discuss.{{/U}} As morphogenesis must be basically the result of changes in behavior of the individual cells, it seems logical to ask morphologists to describe the morphogenetic events observed in terms of changes in cellular contact, changes in the rate of proliferation of cells, or similar phenomena. Once this is done, it may be appropriate to ask questions about the molecular background for these changes. One may, for instance, ask whether variations in cell contact reflect alterations in the populations of molecules at the cell surface, or one may inquire about the molecular basis for the increased cell mobility involved in cell dispersion. Studies of this kind have been carried out with cells released from tissues in various ways and then allowed to reveal their behavior after being spread out into a thin layer. In many cases, such cells show the ability to reaggregate, after which different cell types may sort themselves out into different layers and even take part in still more intricate morphogenetic events. But in most cases, the behavior of cells in the intact embryo is difficult to study because of the thickness and opacity of the cell masses. The sea urchin embryo, however, has the advantage that it is so transparent that each cell can be easily observed throughout development. Thus, {{U}}by recording the development of a sea urchin embryo with time-lapse photography, the research scientist might discover previously unknown features of cellular behavior. Perhaps the study of the sea urchin in this manner can provide a medium by which the molecular biologist and the morphologist can begin communicating with each other more effectively about the way in which genes control morphogenesis.{{/U}}
【正确答案】众所周知,在分子层面上发生的生物变化具有形态发生后果,这些后果影响着组织与器官的形式与分化。若指出胚胎内的基因突变和生物合成过程的干扰会导致生物体形态方面的悖常现象,这实属多余。然则,尽管人们对分子层面上的原因和后果已知之甚多,且虽然对各种胚胎已积累起了大量化学的和形态学的数据,但我们对于基因是如何控制形态发生的理解,仍然极不充分。或许,造成这种情况的原因之一,是分子生物学家与形态学家说着互不相同的语言。当前者说着信使—RNA和蛋白质分子的构象变化时,后者则谈论着外胚层、内胚层和神经脊。 解决这一两难境地的方法之一,是试图去寻找到某些与形态发生相关的现象,无论是分子生物学家还是形态学家都能理解并探讨。由于形态发生基本上必定是单个细胞的行为变化所致的结果,因此,一种似乎符合逻辑的做法是,请求形态学家依照细胞接触、细胞繁殖率变化、或诸般类似现象来描述所观察到的形态发生事件。一旦完成了这一工作,一种恰当的做法可能是去针对引发这些变化的分子背景提出问题。譬如,我们可以问这样一个问题,即细胞接触这方面的变化是否反映出了位于细胞表层的分子总数的变化;抑或,人们可以去探究在细胞扩散中所涉及到的日益增多的细胞移动的分子基础。 科学家用细胞已进行了此类研究,这种细胞从组织中以不同的方式释放出来,并被允许在扩散开去形成一个薄层以后来揭示其行为。在许多情形中,显示出了再聚积的能力,在此之后,不同类型的细胞可能将自己分门别类地区分开来,但在绝大多数情形中,在完好无损的胚胎中的细胞行为却难于研究,因为细胞团块的厚度与不透明性。但是,海胆胚胎具有这样一个优势,即它是如此之透明,以致于在其整个发育过程中,每个细胞都能轻易得到观察。因此,通过用延时摄影记录海胆胚胎的发育过程,科学研究者可能会揭示此前所未知的细胞行为特征。或许,以这种方式,对海胆进行的研究能提供某种媒介,分子生物学家和形态学家可藉此开始围绕着基因控制形态发生的方式这一问题在彼此间进行更为有效的沟通交流。
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