An army scientist has helped solve the decades-old murder mystery surrounding the last Russian czar.
The bones unearthed in a shallow grave definitely are those of Czar Nicholas II, said Lt. Col (Dr.) Victor Weedn at an Aug. 31 news conference. Weedn heads the Armed Forces DNA Identification Laboratory in Rockville, Md., which is involved in identifying skeletal remains of U. S. service members who served in Vietnam, Korea and World War II.
The attempt to identify the czar presented a special challenge. The armed forces lab was the perfect place to perform the type of genetic testing on old, deteriorating bones that was needed in this case, he said.
Until the announcement, scientists had not been able to say for sure whether the bones were those of the czar.
Russian DNA expert Pavel Ivanov, who with Weedn oversaw a team of U. S. military civilians tasked to identify the remains, reached the same conclusion.
Nicholas and his family were rounded up by the Bolsheviks and executed by firing squad in 1918. Their bodies were dumped into a pool of sulfuric acid 20 miles outside the Ural Mountain city of Yekaterinburg.
The shallow grave was uncovered in 1979. Bone fragments believed to be those of the czar, the Czarina Alexandra and three of their five children were unearthed in 1991.
While investigators were able to positively identify the czarina and the daughters early on, a rare, benign genetic condition that first showed up in his generation did not allow them to make a positive identification of Nicholas II.
Rare mutation the key
In the end, it was that genetic mutation which provided the key to solving the mystery, Weedn said. Nicholas' brother, whose remains were exhumed in July 1994, turned out to have the same mutation in his genetic makeup. It is so rare that it makes the identification absolute, he said.
If Russian authorities accept that finding, it will clear the way for the ceremonial burial of the last emperor of Russia.
But the new evidence did not satisfy all skeptics. Emigre Eugene Magerovsky, a retired Russian military intelligence officer, interrupted the news conference to say he was suspicious of how the bones "suddenly" came to light during the Soviet era.
"The Soviets have always been masters of all kinds of shenanigans," he said. He suggested the investigators may have been given two bones from the same corpse, in which case the DNA would have had to match.
Weedn ruled that out, as the tibia and femur from the same side of each body were used in the testing.
Ivanov, a forensic science professor in charge of identifying the remains of the last czar and his family, brought the femur bones—as well as a blood sample from a living relative— to the Rockville laboratory in June.
Much evidence lost
Years of exposure to minerals in the soil destroyed much of the genetic evidence in the bone, Weedn said. Still, through a painstaking process of grinding up bone, reproducing the genetic material from the dust and comparing the results over and over again, the team was able to reach its conclusion.
One mystery Weedn and Ivanov did not address was that of the czar's daughter, Anastasia. Whether she somehow escaped the Bolsheviks' bullets has been the topic of intense debate for more than half a century. The grave yielded bones from only three of the five daughters. Still unresolved is whether Anastasia or Marie might have survived, along with the sickly heir, Alexis.
Weedn, whose laboratory has tested two women who claimed to be Anastasia, found they were not. A third who sought testing has not sent in blood samples for testing, he added. On-again, off-again pairing
Weedn was approached by Ivanov four years ago about becoming involved in the DNA testing of Nicholas II. But his team—including some FBI experts—was replaced by another team of top civilian American forensic scientists. In addition, the British Forensic Science Service in 1992 determined there was a 98.5 percent probability the bones were the czar's.
Weedn and Ivanov's paths crossed again two years later, when another joint investigation was proposed. But that, too, failed to materialize, Weedn said.
Finally, earlier this year, the Russians approached the Armed Forces Institute of Pathology and asked whether Ivanov could come and work with Weedn at the Rockville laboratory.
"There is no question that the greatest experience in DNA identification of old skeletal remains is here at the Armed Forces DNA Lab," Weedn said.