单选题下面关于多克隆位点的描述,不正确的是( ) A.仅存在于质粒载体中 B.具有多种限制性核酸内切酶的识别序列 C.不同限制性核酸内切酶的识别序列可以有重叠 D.人工合成后添加到载体中 E.序列较短
单选题APC是哪种肿瘤的抑癌基因
单选题逆转录酶催化的反应是
A.DNA→DNA
B.DNA→RNA
C.RNA→蛋白质
D.RNA→RNA
E.RNA→DNA
单选题A.甲硫基B.羟基C.巯基D.亚氨基E.咪唑基
单选题信号肽的特点不包括:
单选题原核生物和真核生物中的rRNA具有的相同结构是
A.18SRNA
B.5SrRNA
C.5.8SrRNA
D.28SrRNA
E.16SrRNA
单选题外源基因在大肠杆菌中的高效表达受到很多因素影响,其中SD序列的作用是______。 A.提供一个mRNA转录终止子 B.提供一个mRNA转录起始子 C.提供一个核糖体结合位点 D.提供翻译的终点
单选题能出现在蛋白质分子中的下列氨基酸,哪一种没有遗传密码子
单选题关于PCR的基本成分叙述错误的是
A.特异性引物
B.耐热性DNA聚合酶
C.dNTP
D.含有Zn2+的缓冲液
E.模板
单选题针对配基的生物学特异性的蛋白质分离方法是______。
单选题基因工程技术中实现目的基因与载体DNA连接的酶是( )。
单选题下列哪项不属于真核生物基因的顺式作用元件______
A.激素反应元件
B.衰减子
C.启动子
D.沉默子
E.增强子
单选题体内生成核糖的主要途径是( )
单选题2003年诺贝尔化学奖授予研究生物膜上通道蛋白的科学家,他们阐明了______。
单选题识别碱基改变的感应因子是( ) A.ATM B.ATR C.Chk1 D.Chk2 E.Cdc25A
单选题理想冠桩外形的要求是
单选题RNAi技术的基本程序主要为
A.siRNA对目标RNA的干扰、确定干扰靶点、合成siRNA
B.确定干扰靶点、合成siRNA、siRNA对目标RNA的干扰
C.siRNA对目标RNA的干扰、合成siRNA、确定干扰靶点
D.确定干扰靶点、siRNA对目标RNA的干扰、合成siRNA
E.合成siRNA、确定干扰靶点、siRNA对目标RNA的干扰
单选题严重肝病时,机体各器官会发生出血倾向,主要原因是
A.维生素A减少
B.维生素B6减少
C.维生素C减少
D.维生素E减少
E.维生素K减少
单选题Prince Klemens Von Metternich, foreign minister of the Austrian Empire during the Napoleonic era and its aftermath, would have no trouble recognizing Google. To him, the world's most popular web-search engine would closely resemble the Napoleonic France that in his youth humiliated Austria and Europe's other powers. Its rivals—Yahoo!, the largest of the traditional web gateways, eBay, the biggest online auction and trading site, and Microsoft, a software empire that owns MSN, a struggling web portal—would look a lot like Russia, Prussia, and Austria. Metternich responded by forging an alliance among those three monarchies to create a "balance of power" against France. Google's enemies, he might say, ought now to do the same thing. Google announced two new conquests on August 7th. It struck a deal with Viacom, an "old" media firm, under which it will syndicate video clips from Viacom brands such as MTV and Nickelodeon to other websites, and integrate advertisements into them. This makes Google the clear leader in the fledgling but promising market for web-video advertising. It also announced a deal with News Corporation, another media giant, under which it will pro-vide all the search and text-advertising technology on News Corporation's websites, including My Space, an enormously popular social-networking site. These are hard blows for Yahoo! and MSN, which had also been negotiating with News Corporation. Both firms have been losing market share in web search to Google over the past year—Google now has half the market. They have also fallen further behind in their advertising technologies and networks, so that both make less money than Google does from the same number of searches. Safa Rashtchy, an analyst at Piper Jaffray, a securities firm, estimates that for every advertising dollar that Google makes on a search query, Yahoo! makes only 60-70cents. Last month Yahoo! said that a new advertising algorithm that it had designed to close the gap in profitability will be delayed, and its share price fell by 22% , its biggest-ever one-day drop. MSN is further behind Google than Yahoo! in search, and its parent, Microsoft, faces an even more fundamental threat from the expansionist new power. Many of Google's new ventures beyond web search enable users to do things free of charge through their web browsers that they now do using Microsoft software on their personal computers. Google offers a rudimentary but free online word processor and spreadsheet, for instance. The smaller eBay, on the other hand, might in one sense claim Google as an ally. Google's search results send a lot of traffic to eBay's auction site, and eBay is one of the biggest advertisers on Google's network. But the relationship is imbalanced. An influential re-cent study from Berkeley's Haas School of Business estimated that about 12% of eBay's revenues come indirectly from Google, whereas Google gets only 3% of its revenues from eBay. Worst of all for eBay, Google is starting to undercut its core business. Sellers are setting up their own websites and buying text advertisements from Google, and buyers are using its search rather than eBay to connect with sellers directly. As a result, "eBay would be wise to strike a deep partnership with Yahoo! or Microsoft in order to regain a balance of power in the industry," said the study's authors, Julien Decot and Steve Lee, sounding like diplomats at the Congress of Vienna in 1814.
单选题
真核起始因子eIF-3的作用是:
A、帮助形成亚基起始复合物(eIF-3,GTP,Met-tRNA,40S)
B、帮助亚基起始复合物(三元复合物,40S)与mRNA5′端的结合
C、若与40S亚基结合,防止40S与60S亚基的结合
D、与mRNA5′端帽子结构相结合以解开二级结构
E、激活核糖体GTP酶,使亚基结合可在GTP水解时结合,同时释放eIF-2
