问答题人GM-CSF是由127个氨基酸组成的生长因子，能够调节成熟的粒细胞和巨噬细胞的生物功能，GM-CSF能够治疗髓性再生障碍、粒白细胞低下、化学治疗或放射治疗引起的骨髓造血抑制等疾病治疗药物。人GM-CSFcDNA编码序列中G／C含量为81．5％。本实验室拟用大肠杆菌为表达体系，构建重组人GM-CSF高效表达菌株，以实现GM-CSF的基因工程生产。请你设计实验流程，实现人GM-CSF的高效表达和验证，并请简要注明各关建流程环节的目的或依据。

问答题(中国药科大学2006年考研试题)ketosis

问答题Children in any society are expected to learn to conform to a number of social rules and expectations if they are to become participants in the culture. (46)Among the rules that children in our society are expected to learn are that certain classes of adults (such as teachers and doctors) are addressed by titles, that males and females use separate restroom facilities, and that women but not men wear dresses. These are examples of social conventions. In the absence of such a shared norm, the acts are neither right nor wrong. For this reason, conventions may be said to be arbitrary. For example, (47)we could just as easily have students address teachers by first names as have them call teachers by their last names and formal titles of Mr. or Ms. Conventions, however, serve an important function by providing predictability and order to social life. (48)Without social conventions it would be impossible to organize social institutions such as schools, and societies as organized systems could not exist. The arbitrariness of conventions makes their importance difficult for children to figure out. (49) It is not until some time in adolescence that children come to fully understand the function that these arbitrary conventions serve to provide predictability and order to our social interactions. In contrast with issues of convention are matters of morality. Morality refers to issues of human welfare, justice, and rights that are a function of the inherent features of interpersonal relations. Because of this, the right and wrong of moral actions are not simply determined by social consensus or the views of authority. For example, it is not possible to hit another person with force and not hurt the other person. Similarly, it is not possible to steal something valuable from someone else and not cause the person to experience the sense of loss. A moral judgment about unprovoked harm ( "It is wrong to hit") would not be dependent on the existence of a socially agreed upon rule or standard but could be generated solely from the intrinsic effects of the act (i.e., hitting hurts). (50)Similar analyses could be done regarding a broader range of issues that would extend beyond direct harm to concerns for what it means to be just, compassionate, and considerate of the rights of others.

问答题阻遏

问答题DNA热变性有何特点?T m值表示什么?

问答题简述cAMP活化蛋白激酶的分子机制。

问答题间隔DNA(spacer DNA)

问答题(华中师范大学2010年考研试题)简述蛋白质生物合成的大致步骤和所需的酶或蛋白因子。

问答题DNA连接酶(DNA ligase)

问答题(上海交通大学2006年考研试题)ELISA

问答题简述转座因子(transposable element)的主要类型及特点，你认为转座因子在遗传学上有哪些应用价值?

问答题如果你是一家生物技术公司的CEO或者负责技术的高层人员，有人向你推荐一种治疗癌症的特效药，寻求与你们公司合作。你将在哪些方面对这种药物进行评价?

问答题拓扑异构酶(topoisomerase)

问答题什么是正调控与负调控，试举例说明。

问答题2，3-BPG支路(2，3-diphosphoglycerate bypass)

问答题常染色体上三个隐性基因(a，b，c)是纯合的品系，与其相对野生型的等位基因是A，B，C的纯合的品系进行杂交，F1代雄性个体与隐性纯合的雌性个体进行杂交，得下列结果： 表型 数目 abc 211 ABC 209 aBc 212 AbC 208 试问：哪些基因是连锁的?连锁基因之间的重组值是多少?

问答题比较基因组学(comparative genomics)

问答题(郑州大学2008年、江南大学2007年考研试题)糖酵解

问答题什么是遗传密码子偏好?它是如何影响外源基因在宿主细胞中的表达量的?

问答题放线菌素D能够抑制原核生物的基因转录，却不能用作抗菌药物，试述原因。