摘要
从滇池分离得到水华束丝藻藻株,以BG11为基础培养基,在(25±1)℃,光照强度20μE/m2.s,光/暗周期为16h:8h的培养条件下,水华束丝藻的生长周期约为28d,以光密度(OD665)为指标的生长曲线符合典型的"S"型;在实验浓度范围内,N、P的浓度变化均对水华束丝藻的生长产生影响,在一定范围内([NO3--N]:1.6—245.1 mg/L;[PO43--P]:0.3—1.4 mg/L),高浓度的N、P有利于水华束丝藻的生长,但浓度过高([NO3--N]>245.1 mg/L;[PO43--P]>6.9 mg/L)时,则对其生长产生抑制作用;N、P在缺乏和浓度很低时可对水华束丝藻光系统Ⅱ活性(Fv/Fm)产生显著影响(p<0.01);本实验结果还表明,N、P浓度的改变可影响水华束丝藻硝酸还原酶、酸性磷酸酶和碱性磷酸酶的活性;在低N、P浓度下,水华束丝藻的硝酸还原酶和酸性磷酸酶活性升高,显著高于正常BG11培养时的活性(p<0.05)。这一结果表明,在低N、P营养环境中,水华束丝藻可通过调节自身的酶系统来提高对营养的吸收和利用,从而满足自身生长和细胞增殖的需要。
Aphanizomenon flos-aquae（A.flos-aquae） isolated from eutrophic Lake Dianchi was cultured in BG11 medium,and it was maintained at（25±1）℃（with an illumination of 20 μE/m^2·s,under a light/dark cycle of 16：8）.The growth cycle of A.flos-aquae was approximately 28d and the growth curve was fit well with the typical ＂S＂ model.Concentrations of NO3^--N and PO4^3--P may affect the growth of A.flos-aquae with better growth under higher concentration of N and P,but excessive N and P may restrain the growth of A.flos-aquae.Fv/Fm of A.flos-aquae can only affected by the absence or unusually low contents of N and P.Nitrate reductase,acid phosphatase under low concentrations of N and P were significantly higher than that of the control（cultured in BG11 medium,p〈0.05）.Activities of nitrate reductase and acid/alkaline phosphatase were not linear with the changes of N and P concentration.These results indicated that A.flos-aquae could adapt to variant circumstances with different nutritional levels due to their physiological and biochemical strategies.
出处
《水生生物学报》
CAS
CSCD
北大核心
2007年第6期774-779,共6页
Acta Hydrobiologica Sinica
基金
国家973计划[2002CB412300]
国家重大科技专项(2002AA601011)资助
关键词
蓝藻水华
水华束丝藻
氮
磷
硝酸还原酶
酸性/碱性磷酸酶
光合效能
Cyanobacterica bloom
A.flos-aquae
Nitrate
Phosphorus
Nitrate reductase
Acid/Alkaline phosphatase
Photosynthetic efficiency
