摘要
目的研究孕妇血清25(OH)D水平、VDR-FokI基因多态性与骨密度的关系,探讨孕期检测VDR基因多态性的临床价值。方法随机抽取2018年1月至2019年12月于温州市中心医院产检的351例孕妇作为研究对象,收集临床资料,取外周血用焦磷酸测序法检测VDR-FokI基因多态性,用化学发光法测血清25(OH)D水平,用超声测孕妇桡骨远端及同侧跟骨骨密度,将所得数据用适当的统计学方法进行处理分析。结果351例孕妇中,VDR-FokI FF、Ff、ff基因型分别为85例(24.2%)、172例(49%)和94例(27%);FF、Ff、ff基因型孕妇血清25(OH)D水平分别为(34.41±16.64)nmol/L、(31.44±14.4)nmol/L和(30.25±11.99)nmol/L,三种基因型维生素D水平无明显差异(P>0.05);总体中共有198例(56%)发生骨量异常,其中FF、Ff、ff基因型孕妇骨量异常的人数分别为50例(58%)、96例(59%)、52例(55%)。将总体分成骨量异常组与骨量正常组,结果示两组孕妇FF、Ff、ff基因型构成无明显差异(P>0.05);骨量异常组维生素D明显低于骨量正常组(25.07±9.26nmol/L vs 40.59±15.22 nmol/L)(P<0.05),血清钙无明显差异(2.15±0.18 vs 2.16±0.17mmol/L)(P>0.05)。结论孕妇维生素D水平普遍不足,孕期骨量异常与血清25(OH)D水平有显著相关性,与VDR-FokI基因多态性无明显相关性,正常妊娠孕妇检测VDR-FokI基因多态性临床价值不大。
Objective:To investigate the association among maternal serum vitamin D level,VDR-FokI gene polymorphisms and risks of bone mass density.Methods:During the period of January 2018 to December 2019,351 pregnant women with clear clinical datas collected were randomly selected from Wenzhou central hospital for the study.VDR-FokI gene polymophysms were detected by pyrosequencing,maternal serum 25 hydroxyvitamin D level were detected by chemiluminescence,bone mineral density of distal radius and ipsilateral calcaneus of pregnant women were measured by ultrasound,proper statistics methods were used to process and analyze all the obtained dates.Results:Among all the 351 pregnant women,the number of VDR-FokI genotypes detected in FF,Ff and ff genotypes were 85(24.2%),172(49%)and 94(27%),respectively;The serum 25(OH)D level of pregnant women with FF,Ff and ff genotypes were(34.41±16.64)nmol/L,(31.44±14.4)nmol/L and(30.25±11.99)nmol/L,respectively,and there were no significant differences among the three groups(P>0.05);There were 198 cases(56%)with abnormal bone mass density,among them,50(58%)cases were with FF genotypes,96 cases(59%)with Ff genotypes and 52 cases(55%)with ff genotypes.The FokⅠgene polymophysms in these 198 pregnant women with abnormal bone mass were compared with the rest 153 pregnant women with normal bone mass,and the results showed that there was no significant difference in FF,FF and FF genotype composition between the two groups(P>,0.05).Vitamin D level in the group with abnormal bone mass density was significantly lower than that in the group with normal bone mass density(25.07±9.26nmol/L vs 40.59±15.22nmol/L)(P<0.05);Serum calcium was not significantly different in these two groups(2.15±0.18 vs 2.16±0.17mmol/L)(P>0.05).Conclusion:Vitamin D level in pregnant women is generally deficient,present study confirms that insufficient serum 25(OH)D levels in cases have major contribution to abnormal bone mass density.VDR Fok I polymorphisms did not have any significant association with abnormal bone mass density in pregnant women,Therefore,detection of VDR-FokI gene polymorphism in normal pregnant women is of little clinical value.
作者
王智慧
曾江忠
李苑艳
WANG Zhi-hui;ZENG Jiang-zhong;LI Yuan-yan(Department of Gynecology and Obstetrics,Wenzhou Central Hospital,Wenzhou 325000,China)
出处
《中国优生与遗传杂志》
2020年第7期837-840,共4页
Chinese Journal of Birth Health & Heredity
基金
温州市科技计划项目(Y20170131)
