摘要
目的分析、总结慢性萎缩性胃炎(chronic atrophic gastritis,CAG)动物模型复制的要素,为探讨临床CAG发病过程及有效评价受试药物提供参考依据。方法以“慢性萎缩性胃炎”和“动物”为主题,检索中国期刊全文数据库(CNKI)、万方数据库中近12年相关文献,通过整理纳入文献的实验动物的种类、性别、模型复制方法、周期、阳性药、检测指标等,建立数据库进行统计分析。结果CAG模型实验动物多选用雄性SD大鼠(113次,50.45%)和Wistar大鼠(106次,47.32%);应用自由饮用N-甲基-N’-硝基-N-亚硝基胍(MNNG)、自由饮用0.1%氨水、雷尼替丁溶液灌胃或雷尼替丁饲料喂养、2%水杨酸钠溶液灌胃、30%~40%乙醇灌胃、热生理盐水灌胃、饥饱失常法喂养、夹尾刺激等两种或多种方法的多因素综合诱导法是最常见的模型复制方法(191次);在含有阳性对照药的研究中,应用最多的是维酶素(90次)、胃复春(38次);其中维酶素给药时间最多的两个时间点(段)是28~30 d(28次)和56 d(31次),共计频数59次;中药胃复春给药时间最多的两个时间点(段)是28~30 d(13次)和84 d(9次),共计频数22次。涉及明确证候模型的研究中,出现最多的是脾虚证和肝郁脾虚证;指标检测侧重于胃组织病理法和一般状态观察法。结论现有CAG动物模型多采用多因素综合干预法进行模型复制,选用雄性SD/Wistar大鼠作为实验动物,周期12周,能提高CAG模型成功率;阳性对照药选择维酶素或胃复春,给药时间在30 d,能更好地发挥药物治疗效果;建议综合胃组织病理、一般状况、血清组织生化指标等多种结果进行模型评价。目前缺少统一的动物模型制备及评价标准,因此还需进一步研究,为未来CAG疾病的研究及新药开发提供动物模型基础。
Objective To analyze and summarize the elements for replication of animal model of chronic atrophic gastritis(CAG),and to provide a reference basis for exploring the clinical pathogenesis of CAG and effectively evaluating the tested drugs.Methods Using“chronic atrophic gastritis”and“animals”as the theme,we searched relevant literatures published in the past 12 years from China National Knowledge Internet(CNKI)and Wanfang database.The database was established for statistical analysis by collating animal species,sex,method of model replication,period,positive drug and test index,etc.Results The experimental animals of the CAG model were mostly male SD rats(113 times,50.45%)and Wistar rats(106 times,47.32%).Multifactor combined induction methods,which were established through two or more following ways:free N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)intake,free 0.1%ammonia intake,gavage of Ranitidine solution,feeding of ranitidine,gavage of 2%sodium salicylate solution,gavage of 30%~40%ethanol,gavage of hot saline,feeding of hunger and satiety disorder,tail-clamping stimulation,were the most common methods for model replication(191 times).In the studies of positive control drugs-containing,Vitacoenzyme(90 times)and Wei Fu Chun Capsules(38 times)are frequently applied positive drugs.Vitacoenzyme administration mostly appeared at 28-30 days(28 times)and 56 days(31 times),total frequency was 59 times.Wei Fu Chun Capsules administration mostly appeared at 28-30 days(13 times)and 84 days(9 times),total frequency was 22 times.In the studies involving clear syndromes models,the common syndromes are spleen deficiency syndrome and liver depression and spleen deficiency syndrome.Index tests focused on gastric histopathology and general state observation.Conclusions Existing CAG animal models were replicated using the multi-factor integrated intervention method.Male SD/Wistar rats were selected as experimental animals and treated for 12 weeks,which can improve the success rate of CAG model.Vitacoenzyme or Wei Fu Chun Capsules was used as the positive control drug and the administration lasted for 30 days,which can efficiently exert therapeutic effect.It is recommended to integrate the results of gastric histopathology,general status and serum tissue biochemical indexes for model assessment.However,there is a lack of unified standards for the preparation and evaluation of animal models,so further studies are needed to provide the basis for research on both disease control and development of new drugs for CAG.
作者
葛君玺
秦格
谢逸轩
苗明三
白明
GE Junxi;QIN Ge;XIE Yixuan;MIAO Mingsan;BAI Ming(Henan University of Chinese Medicine,Zhengzhou 450046 Henan,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2023年第6期818-823,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(82274119)
岐黄学者项目(国中医药人教函2022-6)
河南省重大公益专项(201300310100)
关键词
慢性萎缩性胃炎
动物模型
数据挖掘
模型复制要素
chronic atrophic gastritis
animal model
data mining
elements for modeling replication
