摘要
目的:建立藏药粗壮秦艽和麻花秦艽的UPLC-TOF-MS化学成分谱分析方法以及马钱苷酸、龙胆苦苷、獐牙菜苦苷、獐牙菜苷的含量测定方法。方法:依据高分辨质谱提供的分子式、二级质谱裂解碎片及相关文献记载鉴定化学成分。选择[M-H]^(-)作为马钱苷酸的专属离子、[M+HCOO]^(-)作为獐牙菜苦苷、龙胆苦苷、獐牙菜苷专属离子对其进行含量测定,对不同批次药材中化学成分谱进行比较分析。结果:建立了藏药粗壮秦艽和麻花秦艽药材的UPLC-TOF-MS成分图谱,指认38个成分,包括三萜类10个、黄酮类4个、环烯醚萜类21个、其他类成分3个。在2种药材中马钱苷酸、龙胆苦苷、獐牙菜苦苷、獐牙菜苷的含量分别为0.51%~0.90%、1.63%~2.31%、0.08%~0.21%、0.09%~0.28%。结论:建立的藏药粗壮秦艽和中药麻花秦艽UPLC-TOF-MS成分谱能全面反映药材的化学成分组成以及不同基原药材的成分差异,含量测定方法稳定可行,可为藏药粗壮秦艽药材的质量评价提供参考。
Objective:To establish chemical composition chromatogram of Gentiana robusta and Gentiana straminea roots and determine the contents of loganic acid,gentiopicroside,swertiamarin,sweroside by UPLC-TOF-MS technique.Methods:The chemical compositions were identified according to the molecular formula,secondary mass spectrometry fragmentation provided by high resolution mass spectrometry and related literature records.The contents of loganic acid was determined by using[M-H]^(-)ion,and[M+HCOO]^(-)was used as the exclusive ion for the determination of gentiopicroside,swertiamarin,sweroside.The chemical composition spectra of different batches of medicinal materials were compared and analyzed.Results:The chemical composition chromatogram of Gentiana robusta and Gentiana straminea roots was established and thirty-eight chemical constituents in the chromatogram were identified,including 10 triterpenes,4 flavonoids,21 iridoids and 3 other components.The contents of loganic acid,gentiopicroside,swertiamarin,sweroside were 0.51%to 0.90%,1.63%to 2.31%,0.08%to 0.21%,0.09%to 0.28%,respectively.Conclusion:The established UPLC-TOF-MS composition spectra of Gentiana robusta and Gentiana straminea roots are able to fully reflect the chemical compositions and the composition differences.The content determination method is stable and feasible,which can provide reference for the quality evaluation of Gentiana robusta roots.
作者
熊波
夏云
倪梁红
桑吉东知
杨晓露
沈伟
范蓓蓓
XIONG Bo;XIA Yun;NI Liang-hong;SANGJI Dong-zhi;YANG Xiao-lu;SHEN Wei;FAN Bei-bei(Department of Pharmacy,Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 201999,China;School of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Pharmacognosy,Tibetan Hospital of Tibet Autonomous Region,Lhasa 850000,China)
出处
《中药材》
CAS
北大核心
2023年第2期407-413,共7页
Journal of Chinese Medicinal Materials
基金
国家自然培育基金项目(GZRPYJJ-201805)
北京医卫健康公益基金会医学科学研究基金项目(YWJKJJHKYJJ-B20210CS)
关键词
粗壮秦艽
麻花秦艽
环烯醚萜苷
UPLC-TOF-MS
成分图谱
Gentiana robusta King ex Hook.f.
Gentiana straminea Maxim.
Iridoid glycosides
UPLC-TOF-MS
Chemical composition chromatogram