摘要
目的:比较不同采收季节怀牛膝HPLC指纹图谱,探讨采收季节对怀牛膝内部质量的影响。方法:采用超声提取法制备样品,建立HPLC指纹图谱;运用相似度评价、灰色关联度分析、主成分分析法等研究不同采收季节怀牛膝的质量,并比较其差异。结果:冬季、春季采收季节怀牛膝指纹图谱共有模式分别标定了21、22个共有峰,相似度分别为0.994~1.000、0.997~1.000,两个季节采收的怀牛膝均含有β-蜕皮甾酮、25R-牛膝甾酮、25S-牛膝甾酮;差异分析结果显示,色谱峰1是冬季采收怀牛膝的特征峰,色谱峰4是春季采收怀牛膝的特征峰。灰色关联度分析和主成分分析结果均表明不同采收季节怀牛膝质量为春季>冬季。结论:不同采收季节怀牛膝化学成分含量及种类存在差异,根据本研究结果,建议采收季节为春季。
Objective:To compare the HPLC fingerprint of Achyranthes bidentata in different harvesting seasons,in order to explore the influence of harvesting seasons on internal quality of Achyranthes bidentata.Methods:Samples were prepared by ultrasonic extraction and HPLC fingerprint was established;The quality of Achyranthes bidentata in different harvesting seasons was studied by similarity evaluation,grey correlation analysis and principal component analysis and the differences were compared.Results:Twenty-one and twenty-two common peaks were demarcated by fingerprint pattern of Achyranthes bidentata harvested in winter and spring,respectively.The similarity ranged from 0.994 to 1.000 and 0.997 to 1.000,respectively.There wereβ-ecdysterone,25R-inokosterone and 25S-inokosterone in Achyranthes bidentata collected in two seasons.The results of differential analysis showed that the chromatographic peak 1 was the characteristic peak of Achyranthes bidentata in winter and peak 4 was the characteristic peak in spring.The results of grey correlation analysis and principal component analysis showed that the quality of Achyranthes bidentata in spring was better than that in winter.Conclusion:The contents and types of chemical constituents of Achyranthes bidentata are different in different harvesting seasons.According to the results of this study,the harvest season is suggested to be spring.
作者
唐维维
梁献葵
马驰虹
雷敬卫
龚海燕
纪亮
谢彩侠
TANG Wei-wei;LIANG Xian-kui;MA Chi-hong;LEI Jing-wei;GONG Hai-yan;JI Liang;XIE Cai-xia(Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中药材》
CAS
北大核心
2019年第9期2079-2085,共7页
Journal of Chinese Medicinal Materials
基金
国家重点研发计划“中医药现代化研究”重点专项(2018YFC1707000)
河南省科技攻关计划项目(142102313109)
关键词
怀牛膝
指纹图谱
灰色关联度分析
主成分分析
Achyranthes bidentata Bl.
Fingerprint
Grey correlation analysis
Principal component analysis
