摘要
近些年链格孢属真菌的分类有了长足的进展,但柑橘褐斑病和黑腐病的病原却还是存在着一些争议。本研究从中国柑橘果实上收集了49株黑腐病菌菌株,并选取了8株从柑橘叶片上分离的具代表性的褐斑病菌菌株,基于Alta1、endoPG、LSU、OPA10-2、OPAl-3和OPA2-1等6个基因位点串联序列构建系统发育树。结果表明,柑橘黑腐病和褐斑病都可由不止一种链格孢菌引起,均以Alternaria alternata为主。两种病害的病原菌之间不能通过该系统发育树区分,但在致病性上存在差异,且能通过扩增ACT毒素合成基因进行区分。为了使两种病害的病原更加方便阐述,作者建议以它们的主要类群对其进行命名,柑橘褐斑病病原学名还是遵循前人的称呼,为the tangerine pathotype of A.alternata,即链格孢菌橘致病型,而柑橘黑腐病病原应为A.alternata,即链格孢菌。
The research on the classification of Alternaria has made rapid progress in recent years,while the exact pathogen species responsible for Alternaria brown spot and Alternaria black rot remains to be identified.In this research,49 strains were isolated from citrus fruits with symptoms Alternaria black rot,while 8 representative strains from citrus leaves with symptoms of Alternaria brown spot.Phylogenetic trees were constructed on the basis of the combined sequences of Alta1,endoPG,LSU,OPA10-2,OPAl-3 and OPA2-1.The study showed that both Alternaria black rot and Alternaria brown spot could be caused by more than one species of Alternaria in which Alternaria alternate is the predominant.The pathogen species of these two diseases cannot be distinguished by the phylogenetic tree,but the pathogenicity as well as ACT toxin synthesis gene can be used to differentiate isolates into groups.The two pathogenic species associated with the diseases were proposed to be named after their predominant species.The pathogen causing Alternaria brown spot should keep the original name,the tangerine pathotype of A.alternate.Accordingly,the pathogen causing Alternaria black rot should be called as A.alternata.
作者
张斌
梅秀凤
黄峰
王明爽
王洪凯
李红叶
ZHANG Bin;MEI Xiu?feng;HUANG Feng;WANG Ming?shuang;WANG Hong?kai;LI Hong?ye(Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2020年第1期10-19,共10页
Acta Phytopathologica Sinica
基金
国家自然科学基金(31571948)
现代农业(柑橘)产业技术体系(CARS-26)项目资助.
