摘要
目的探讨慢性萎缩性胃炎伴肠上皮化生患者体质类型与肠道菌群相关性。方法以2021年1~8月期间川北医学院附属医院收治的144例慢性萎缩性胃炎伴肠上皮化生患者为研究对象,均于确诊时行肠道菌群检测及体质类型诊断,根据体质类型(病态体质、和平体质)分为病态体质组和和平体质组。观察病态体质组和和平体质组的肠道菌群(各菌属类群相对丰度、各菌门类群相对丰度、菌群多样性(Chao1指数、Shannon指数、Sobs指数))差异,并采用Spearman秩相关法分析体质类型(是否病态体质)与肠道菌群指标的相关性。结果病态体质组和和平体质组在肠道菌群门类和属类的主成分上存在显著差异(R=-8.021,P=0.008)。病态体质组的Sobs指数、Chao指数、Shannon指数均低于和平体质组(t=9.637~18.715,P均<0.05);体质分型(是否病态体质)与肠道菌群Chao1指数(r=-0.718,P=0.016)、肠道菌群Shannon指数(r=-0.749,P=0.001)、肠道菌群Sobs指数(r=-0.694,P=0.016)均呈负相关。病态体质组的有益菌(双歧杆菌属、普氏菌属、另枝菌属)相对丰度低于和平体质组,病态体质组的有害菌Dorea相对丰度高于和平体质组(t=10.448~19.031,P均<0.05);体质分型(是否病态体质)与双歧杆菌属相对丰度(r=-0.663,P=0.016)、普氏菌属相对丰度(r=-0.718,P=0.014)、另枝菌属相对丰度(r=-0.709,P=0.016)均呈负相关,体质分型(是否病态体质)与有害菌Dorea相对丰度(r=0.649,P=0.017)呈正相关。病态体质组的厚壁菌门低于和平体质组(t=6.714,P均<0.05);体质分型(是否病态体质)与厚壁菌门相对丰度(r=-0.718,P=0.012)呈负相关。结论慢性萎缩性胃炎伴肠上皮化生患者的病态体质与肠道菌群丰度、菌群物种复杂度、菌群群落多样性均有密切的相关性,故肠道菌群丰度、复杂度、多样性的控制可能利于慢性萎缩性胃炎伴肠上皮化生患者的病态体质防治。
Objective To investigate the correlation of constitution type and intestinal flora in chronic atrophic gastritis with intestinal metaplasia.Methods 144patients with chronic atrophic gastritis with intestinal metaplasia admitted to the Affiliated Hospital of North Sichuan Medical College from January 2021to August 2021were selected as the research object.Intestinal flora testing and physical type diagnosis were performed at the time of diagnosis,according to physical type(Sick constitution and peaceful constitution)are divided into sick constitution group and peaceful constitution group.The differences of intestinal flora relative abundance of genera,phyla,flora diversity(Chao1index,Shannon index,Sobs index)between pathological and peaceful constitution groups,and analyze the correlation between constitution type(pathological constitution)and intestinal flora index by Spearman rank correlation method were observed.Results There were significant differences in the principal components of intestinal flora between Morbid and peaceful constitution groups(R=-8.021,P=0.008).Sobs Index,Chao Index and Shannon index in Morbid Constitution group were lower than those in peace constitution group(t=9.637~18.715,P<0.05)There was a negative correlation between body Constitution and CHAO1index(R=-0.718,P=0.016),Shannon Index(r=-0.749,P=0.001),Sobs index(r=-0.694,P=0.016).The relative abundance of beneficial bacteria(bifidobacterium,Prevotella and other mycobacteria)in ill constitution group was lower than that in peace constitution group,and the relative abundance of harmful bacteria(Dorea)in ill constitution group was higher than that in peace constitution group(t=10.448~19.031,P<0.05)The relative abundance of Bifidobacterium(R=-0.663,P=0.016),Prevotella(R=-0.718,P=0.014)and other cladosporium(r=-0.709,P=0.016)were all negatively correlated with the constitution type,there was a positive correlation between the relative abundance of Dorea(r=0.649,P=0.017)and the constitution type.Pachyderm in Morbid Constitution Group was lower than that in peace constitution group(t=6.714,P<0.05),and there was a negative correlation between constitution type and relative abundance of Pachyderm(R=-0.718,P=0.012).Conclusion The pathological constitution of patients with chronic atrophic gastritis with intestinal metaplasia is closely related to the abundance of intestinal flora,species complexity and community diversity.Therefore,the control of the abundance,complexity and diversity of intestinal flora may be conducive to the pathological constitution prevention and control of chronic atrophic gastritis with intestinal metaplasia.
作者
杨阳
刘浩
刘洁
YANG Yang;LIU Hao;LIU Jie(Department of Traditional Chinese and Western Medicine Clinical Medicine,North Sichuan Medical College,Nanchon Sichuan 637000,China;Institute of Pharmacology and Toxicologyof Traditional Chinese Medicine,Sichuan Academy of Chinese Medical Sciences,Chengdu Sichuan 610041,China)
出处
《中国实验诊断学》
2023年第8期975-979,共5页
Chinese Journal of Laboratory Diagnosis
基金
四川省软科学研究计划项目(2018ZR0386)
关键词
慢性萎缩性胃炎
肠上皮化生
体质类型
肠道菌群
相关性
chronic atrophic gastritis
intestinal metaplasia
constitution type
intestinal flora
correlation
