摘要
目的探讨镧经口暴露后在大鼠体内的吸收、分布、蓄积、排泄规律以及镧对大鼠血液元素谱的影响。方法48只雌性SPF级大鼠随机分为8组进行单次经口暴露实验,一次性灌胃700 mg/kg·BW硝酸镧溶液。于灌胃后1、2、4、8、16、32、64、128 h采用电感耦合等离子体质谱仪(ICP-MS)测定大鼠全血、心、肝、脾、肺、肾、脑、股骨、粪便镧含量。48只SPF级大鼠随机分为4组(雌雄各半)进行重复经口暴露实验,每组含1个对照组和1个实验组。实验组重复灌胃镧含量66 mg/kg·BW的硝酸镧溶液,对照组灌胃等量生理盐水,每天1次,在第7、14、21和28 d时,各取1组(含对照组和实验组),采用ICP-MS法测定大鼠全血44种元素含量以及肝、肾、股骨、粪便和尿液镧含量。结果大鼠单次经口暴露镧后,镧在全血中达峰时间为2 h,消除半衰期为69.315 h,总体清除率为1.733 L/h/kg;镧随血液广泛分布于心、肝、脾、肺、肾、脑和股骨,以股骨中含量最高(P<0.05);大鼠重复经口暴露镧14、21和28 d后,与肾含量相比,大鼠肝和股骨镧含量显著升高(P<0.05),且实验组大鼠粪便镧含量显著高于尿液(P<0.001)。大鼠重复给予镧处理后,与对照组相比,实验组大鼠全血中除了镧含量显著升高(P<0.05)外,其余元素(钙、钠、钾、镁、铁、铜、锌、硒、钴、钼、铅、砷)含量在对照组和实验组间未见统计学差异(P>0.05)。结论本试验镧经口暴露后可快速吸收入血,并广泛分布于心、肝、脾、肺、肾、脑和股骨,重复暴露后易沉积于肝和股骨,且以粪便排泄为主;未观察到镧对大鼠血液元素谱产生影响。
Objective To explore the absorption,distribution,accumulation,and excretion after oral exposure to lanthanum and the effects of lanthanum on the blood element spectrum of rats.Methods 48 female SPF-grade rats were randomly divided into 8 groups with a one-time gavage of 700 mg/kg·BW lanthanum nitrate solution using single oral exposure experiment.And the blood,heart,liver,spleen,lung,kidneys,brain,femur,and fecal of rats were collected and measured by ICP-MS after gavage for 1,2,4,8,16,32,64,and 128 h.Another 48 SPF-grade rats were randomly divided into 4 groups(half male and female),each group with a control and an experimental group was observed in experimental time-point in repeat oral exposure experiment.The experimental group repeatedly gavage lanthanum nitrate solution containing lanthanum 66 mg/kg·BW,and the control group gavage an equal amount of physiological saline.Then,on the 7th,14th,21st and 28th days,the rats(including the control group and the experimental group)were taken to determine the contents of 44 elements in blood and the contents of lanthanum in liver,kidney,femur,feces and urine by ICP-MS.Results After single oral exposure to lanthanum,lanthanum peaked in blood for 2 h,the elimination half-life was 69.315 h,and the overall clearance rate was 1.733 L/h/kg.Lanthanum was widely distributed in the heart,liver,spleen,lung,kidneys,brain,and femur with blood,and the level in the femur was the highest(P<0.05).The levels of lanthanum in the liver and femur increased compared with kidneys after repeated oral exposure to lanthanum for 14,21,and 28 d(P<0.05),and the level of lanthanum in fecal of rats was higher than that of urine(P<0.001).Compared to the control group,the level of lanthanum increased in the blood of rats(P<0.05),while there was no statistically significant difference between the control group and the experimental group in calcium,sodium,potassium,magnesium,iron,copper,zinc,selenium,cobalt,molybdenum,lead and arsenic in the blood of rats(P>0.05).Conclusion In this experiment,lanthanum can be rapidly absorbed into the blood after oral exposure and is widely distributed in the heart,liver,spleen,lung,kidneys,brain,and femur.After repeat oral exposure to lanthanum,lanthanum is easily deposited in the liver and femur,feces excretion is the main route of elimination,and no effect of lanthanum on the blood element spectrum of rats has been observed.
作者
林秀琴
陈颖思
黎姿茵
徐飞飞
万思雨
赵晓晗
宋佳
吴炜亮
杨杏芬
LIN Xiuqin;CHEN Yingsi;LI Ziyin;XU Feifei;WAN Siyu;ZHAO Xiaohan;SONG Jia;WU Weiliang;YANG Xingfen(Food Safety and Health Research Center,NMPA Key Laboratory for Safety Evaluation of Cosmetics,Guangdong Provincial Key Laboratory of Tropical Disease Research,School of Public Health,Southern Medical University,Guangdong Guangzhou 501515,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2023年第8期1152-1159,共8页
Chinese Journal of Food Hygiene
基金
国家重点研发计划(2022YFF0711100)
广东省药品监督管理局2022年科技创新重点实验室项目(2022ZDZ09)
广东省重点研发计划(2019B020210002)
关键词
稀土元素
硝酸镧
大鼠
代谢动力学
血液元素谱
Rare earth elements
lanthanum nitrate
rats
pharmacokinetics
blood element spectrum
