摘要
目的:筛选黄连-吴茱萸配伍对胃癌治疗的主要活性成分,预测两者共同活性成分的作用靶点,建立药物活性成分-靶基因网络,进一步探讨药对黄连-吴茱萸对胃癌治疗的潜在作用机制。方法:采用中药系统药理学技术平台(TCMSP),筛选黄连-吴茱萸的潜在活性化学物质,借助基因组注释(Genecards)数据库平台,预测和筛选胃癌的作用靶点,采用Cytoscape(3.7.1)软件,构建"药物-活性化学物质-疾病-作用靶点"网络图,借助String数据库平台构建蛋白质相互作用网络。最后利用Bioconductor平台和R语言进行基因本体(GO)富集分析和基因相互作用(KEGG)通路分析。结果:通过筛选,得到黄连的潜在活性成分14个,吴茱萸的潜在活性成分15个,共涉及与胃癌相关作用靶点127个。黄连-吴茱萸-胃癌共同靶点33个,主要通过调控PTGS2,PTGS1,AR,RXRA,NOS2等靶基因及细胞凋亡,p53信号通路,白细胞介素(IL)-17信号通路等发挥对胃癌的治疗作用。结论:黄连-吴茱萸对胃癌的治疗作用体现了中药多成分-多靶点的特点,阐明了药对黄连-吴茱萸对胃癌治疗的作用机制与物质基础的科学内涵。
Objective:To identify the main active components of Coptidis Rhizoma and Euodiae Fructus for the treatment of gastric cancer,predict the targets of the common active components in these two herbs,establish the network of active drug components-target genes,and further explore the potential mechanism and effect of Coptidis Rhizoma-Euodiae Fructus for the treatment of gastric cancer.Method:The active components of Coptidis Rhizoma-Euodiae Fructus were screened by Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),gastric cancer targets were predicted and screened by Genecard database platform,"drug-active ingredient-disease-targets"networks were built by Cytoscape(3.7.1)software,and protein interaction networks were built by String database platform.Finally,Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were performed by using Bioconductor platform and R language.Result:The 14 potential active components of Coptidis Rhizoma and 15 potential active components of Euodiae Fructus were obtained,involving 127 targets related to gastric cancer.There were 33 common targets for Coptidis RhizomaEuodiae Fructus-gastric cancer,which played a therapeutic role in gastric cancer mainly by regulating target genes such as PTGS2,PTGS1,AR,RXRA and NOS2,as well as cell apoptosis,p53 signaling pathway and interleukin(IL)-17 signaling pathway.Conclusion:The therapeutic mechanism of Coptidis Rhizoma-Euodiae Fructus reflects the multi-component,multi-target and multi-pathway characteristics of traditional Chinese medicines and provides the scientific basis for further study and the material basis of Coptidis Rhizoma-Euodiae Fructus against gastric cancer.
作者
郑琦
侯炜
ZHENG Qi;HOU Wei(Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第7期204-210,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81673754).
关键词
黄连
吴茱萸
胃癌
网络药理学
Coptidis Rhizoma
Euodiae Fructus
gastric cancer
network pharmacology
