摘要
本文比较了平板计数法、凝胶型菌落总数测试片法和无纺布型菌落总数测试片法对检测不同类型样品中菌落总数结果的差异。通过比较大肠埃希氏菌、金黄色葡萄球菌和枯草芽孢杆菌标准菌株分别在平板计数琼脂、凝胶型菌落总数测试片和无纺布型菌落总数测试片3种培养基中的菌落形态,并采用这3种方法对2种食品样品Y628和A530进行菌落总数测定和数据分析,确保检验结果的准确性。实验结果显示,凝胶型菌落总数测试片在菌落辨识度上优于平板计数琼脂和无纺布型菌落总数测试片,对于不同样品的检测,结果更易于判读和计数。采用凝胶型菌落总数测试片和无纺布型菌落总数测得的结果与平板计数琼脂测得的结果无显著差异。利用传统平板计数法与市面上不同类型的菌落测试片法对样品Y628和A530的菌落总数进行测定,结果并没有显著性差别,因此菌落总数测试片也可作为上述食品的菌落总数测定。
This paper compares the differences in the results of plate counting method,gel-based colony counting test piece and non-woven colony counting test piece for detecting colony counts in different types of samples.The colony morphology of standard strains of Escherichia coli,Staphylococcus aureus and Bacillus subtilis were compared in three types of media,namely,plate count agar,gel-type colony count test piece and non-woven colony count test piece,respectively.The colony counts of Y628 and A530 food samples were determined by the three methods,and data were analyzed to ensure the accuracy of the test results.The results show that the colony identification of gel colonies is better than that of agar and nonwoven colonies,and the results are easier to read and count in different samples.The results obtained using the total number of gel-type colonies and the total number of non-woven colonies are not significantly different from the results obtained by plate count of agar.There is no significant difference between the traditional plate counting method and different types of colony test tablets on the market to determine the total number of colonies of samples Y628 and A530.Therefore,the colony test piece method can be used to determine the total number of colonies in the food mentioned above.
作者
麻晓莉
MA Xiao-Li(Technical Center of Nanning Customs District,Nanning 530021)
出处
《中国口岸科学技术》
2024年第S01期107-112,共6页
China Port Science and Technology
关键词
菌落总数
平板计数法
菌落测试片
不同类型样品
配对T检验
total count of colony
plate counting
colony test plate
different types of samples
paired t-test
