摘要
目的探讨人乳头状瘤病毒(HPV)E6/E7信使核糖核酸(mRNA)、细胞增殖相关核蛋白(Ki-67)在宫颈上皮内瘤变(CIN)中的表达及临床价值。方法选取2019年3月-2020年3月浙江省宁海县妇幼保健院收治的89例子宫颈病变患者,根据宫颈病变级别进行分组,其中CIN1级组18例、CIN2级组31例、CIN3级组26例和宫颈鳞状细胞癌组14例。选取同期收治的23例子宫良性病变患者为对照组,术中取子宫颈组织作为对照。比较不同组织HPV E6/E7 mRNA和Ki-67表达,分析患者HPV E6/E7 mRNA和Ki-67表达与宫颈病变程度的相关性。结果与对照组比较,CIN1级组、CIN2级组、CIN3级组及宫颈鳞状细胞癌组HPV E6/E7 mRNA、Ki-67阳性表达率升高,差异有统计学意义(P<0.05)。HPV E6/E7 mRNA及Ki-67阳性表达率与宫颈病变程度呈正相关(r_(HPVE6/E7mRNA)=0.712,P=0.003;r_(Ki-67)=0.698,P=0.008)。结论HPV E6/E7 mRNA和Ki-67在子宫颈病变组织中阳性表达率较高,且与病变程度呈正相关,临床可检测这两种指标,以便早期发现宫颈上皮病变。
Objective To investigate the expression and clinical value of human papillomavirus(HPV)E6/E7 messenger ribonucleic acid(mRNA)and cell proliferation-related nuclear protein(Ki-67)in cervical intraepithelial neoplasia(CIN).Methods From March 2019 to March 2020,89 patients with cervical lesions admitted to the Department of Gynecology of Ninghai County Maternal and Child Health Hospital in Zhejiang Province were selected and divided into groups according to the level of cervical lesions,including 18 cases in the CIN1 group,31 cases in the CIN2 group,and CIN3 There were 26 cases in the grade group and 14 cases in the cervical squamous cell carcinoma group.The 23 patients with benign uterine lesions admitted in the same period were selected as the control group,and the cervical tissues were taken as the control group.Compare the expression of HPV E6/E7 mRNA and Ki-67 in different tissues,and analyze the correlation between the expression of HPV E6/E7 mRNA and Ki-67 and the degree of cervical lesions in patients.Results Compared with the control group,the positive expression rates of HPV E6/E7 mRNA and Ki-67 increased in the CIN1,CIN2,CIN3 and cervical squamous cell carcinoma groups,and the difference was statistically significant(P<0.05).The positive expression rate of HPV E6/E7 mRNA and Ki-67 was positively correlated with the degree of cervical lesions(r_(HPV E6/E7 mRNA)=0.712,P=0.003;r_(Ki-67)=0.698,P=0.008).Conclusion The positive expression rate of HPV E6/E7 mRNA and Ki-67 in cervical lesions is relatively high,and it is positively correlated with the degree of lesions.These two indicators can be detected in cliuic to detect cervical epithe lial lesions asearly as possible.
作者
蒋笑霞
李红梅
俞旭云
王福智
JIANG Xiaoxia;LI Hongmei;YU Xuyun;WANG Fuzhi(Ninghai maternal and Child Health Hospital,Ninghai 315600,China)
出处
《浙江实用医学》
2021年第3期206-208,共3页
Zhejiang Practical Medicine
