摘要
基于网络药理学方法探讨"淫羊藿-白芍"药对治疗腰椎间盘突出症的关键靶点及作用机制。利用目前国内外公认的数据库平台和分析软件,从药物和疾病2个方面出发构建网络,利用TCMSP等数据库收集淫羊藿、白芍的化学成分,根据阈值筛选及结合文献报道确定其有效活性成分并预测作用靶点。通过GeneCards,OMIM,DisGeNET数据库收集腰椎间盘突出症疾病基因。将药物靶点与疾病靶点进行映射,并对关键靶点进行蛋白互作网络分析、GO功能富集分析和KEGG信号通路富集分析。最终分别确定淫羊藿和白芍有效活性成分23,13个,通过检索共获得药物靶点624个,经过标准化处理后获得214个药物靶点。通过GeneCards,OMIM和DisGeNET数据库分别收集腰椎间盘突出症的相关靶点306,2,5个,汇总去重后共获得293个疾病靶点。药物靶点与疾病靶点映射后获得44个共有靶点,PPI蛋白互作网络分析发现IL-6,TNF,AKT1,MAPK1,VEGFA等可能是"淫羊藿-白芍"治疗腰椎间盘突出症的核心靶点。GO富集分析确定了56个条目(P<0.05),其中生物过程主要包括免疫反应、细胞凋亡等;细胞成分主要包括细胞外空间、细胞外区域等;分子功能主要包括细胞因子活性、金属肽酶活性等。KEGG通路富集分析确定了91条相关信号通路,其中信号通路涉及炎症、代谢、衰老等方面,主要有IL-17信号通路、TNF信号通路等。"淫羊藿-白芍"药对治疗腰椎间盘突出症具有多途径、多靶点作用的特点。该研究初步探讨了其作用的关键靶点及涉及的生物学过程和信号通路,发现其可能是通过影响炎症、免疫调节等途径发挥治疗作用,为接下来进一步利用分子生物学进行实验验证奠定了基础。
The aim of this paper was to investigate the key targets and mechanism of"Epimedii Folium-Paeoniae Radix Alba"in the treatment of lumbar disc herniation by means of network pharmacology.The currently recognized databases and analysis software at home and abroad were used to construct the network from drugs and diseases.The chemical components of Epimedii Folium and Paeo-niae Radix Alba were collected by using databases such as TCMSP,while their active components were determined and the action targets were predicted according to threshold screening and literature reports.The genes for lumbar disc herniation were collected by using GeneCards,OMIM,and DisGeNET databases.The drug targets were mapped to disease targets,and protein interaction network analysis for key targets,GO function enrichment analysis and KEGG signaling pathway enrichment analysis were performed.Finally,23 active components of Epimedium Folium and 13 active components of Paeoniae Radix Alba were determined,and a total of 624 drug targets were obtained.After standardization,214 drug targets were obtained.In addition,306,2 and 5 related targets of lumbar disc herniation were collected from GeneCards,OMIM,and DisGeNET database,respectively,and a total of 293 disease targets were obtained after deduplication.After the mapping of drug target and disease target,44 common targets were obtained.PPI protein interaction network analysis showed that IL-6,TNF,AKT1,MAPK1,and VEGFA may be the core targets for the treatment of lumbar disc herniation.GO enrichment analysis identified 56 items(P<0.05),among which biological processes mainly included immune response,apoptosis,etc.;cell components mainly included extracellular space,extracellular region,etc.;molecular functions mainly included cytokine activity,metallopeptidase activity and so on.Through KEGG pathway enrichment analysis,91 signaling pathways related to inflammation,metabolism,and senescence were identified,mainly including IL-17 signaling pathway and TNF signaling pathway and so on."Epimedii Folium-Paeoniae Radix Alba"showed the characteristics of multi-channel and multi-target for the treatment of lumbar disc herniation.This study preliminarily explored the key targets for its role and the biological processes and signaling pathways involved.It was found that it may play a therapeutic role by affecting inflammation and immune regulation,which laid the foundation for further experimental verification.
作者
孙凯
朱立国
魏戌
张平
展嘉文
王源
银河
SUN Kai;ZHU Li-guo;WEI Xu;ZHANG Ping;ZHAN Jia-wen;WANG Yuan;YIN He(Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China;Beijing Key Laboratory of Orthopedics of Traditional Chinese Medicine,Beijing 100007,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第3期609-616,共8页
China Journal of Chinese Materia Medica
基金
国家中医药领军人才支持计划——“岐黄学者”计划项目
国家自然科学基金青年基金项目(81704102)
中华中医药学会(2017—2019年度)青年人才托举工程项目(CACM-2017-QNRC2-A03)
中国中医科学院“十三五”重点领域科研项目(ZZ10-022).
关键词
腰椎间盘突出症
网络药理学
“淫羊藿-白芍”药对
作用机制
lumbar disc herniation
network pharmacology
"Epimedii Folium-Paeoniae Radix Alba"drug pair
mechanism
