摘要
黄芪为常用的大宗药材,《中国药典》收载含黄芪的复方制剂大约150首,但《中国药典》黄芪含量测定项下样品制备方法繁琐耗时,不便于大量样本的检验。因此,简化样品制备方法、提高方法的实用性对黄芪及其制剂的质量控制具有重要意义。该研究采用超声提取方法取代加热回流提取,采用固相萃取方法富集制备样品,为黄芪饮片和标准汤剂建立了一套实用的质量评价方法,所建立的方法大大缩短了样品制备时间、提高了方法的准确度。黄芪标准汤剂分析结果显示,黄芪毛蕊异黄酮葡萄糖苷的转移率(96.5±28.7)%变异很大,通过研究发现这与标准汤剂制备过程中,毛蕊异黄酮葡萄糖苷丙二酸酯转化为毛蕊异黄酮葡萄糖苷有关;而毛蕊异黄酮和黄芪甲苷转移率分别为(59.4±14.4)%和(101.3±12.3)%,较为稳定。因此,建议黄芪饮片及水煎液制剂以毛蕊异黄酮和黄芪甲苷作为质量评价指标。该结果为黄芪饮片及其标准汤剂的质量控制建立了一套具有科学性和实用性的分析方法,也为其制剂的质量评价方法的建立提供了科学依据。
Astragali Radix is commonly used as bulk medicinal materials.Chinese Pharmacopoeia contains about 150 compound preparations of Astragali Radix,but the sample preparation method under the determination of Astragali Radix content in Chinese Pharmacopoeia is tedious and time-consuming,not convenient for the test of a large number of samples.Therefore,it is of great significance to simplify the sample preparation method and improve the practicability of the method for the quality control of Astragali Radix and its preparations.In this study,ultrasonic extraction method was used instead of heated reflux extraction,and solid phase extraction method was used to enrich and prepare the samples.A set of practical quality evaluation method was established for Astragali Radix slices and standard decoction,greatly shortening the sample preparation time and improving the accuracy of the method.The results of Astragali Radix standard decoction analysis showed that the transfer rate of calycosin 7-O-β-D-glucospyranoside,(96.5±28.7)%,had great variation,which was found to be related to the conversion of mulberry isoflavone glucoside into calycosin 7-O-β-D-glucospyranoside during the preparation of standard decoction.The transfer rates were(59.4±14.4)%and(101.3±12.3)%for calycosin and astragalosideⅣrespectively,which were relatively stable.Therefore,it is suggested that Astragali Radix slices and water decoction preparations should be evaluated by using calycosin and astragalosideⅣas the quality evaluation index.The results provide a scientific and practical method for quality control of Astragali Radix slices and its standard decoction,and also provide scientific evidence for quality evaluation of the preparations.
作者
王东博
赵曼佳
代云桃
秦雪梅
陈士林
WANG Dong-bo;ZHAO Man-jia;DAI Yun-tao;QIN Xue-mei;CHEN Shi-lin(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijng 100700,China;School of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,China;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第1期119-123,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81473340,81803734)
中国中医科学院中药研究所中央公益性科研院所基本科研业务费项目(ZXKT17009).
关键词
黄芪
标准汤剂
质量传递规律
毛蕊异黄酮葡萄糖苷
毛蕊异黄酮
黄芪甲苷
固相萃取
Astragali Radix
standard decoction
transfer rate
calycosin 7-O-β-D-glucospyranoside
calycosin
astragalosideⅣ
solid phase extraction
