摘要
目的:探讨淫羊藿总黄酮(Epext)对膝关节骨性关节炎的作用及分子机制。方法:将60只雄性SD大鼠分为假手术组、模型组、Epext(600,300,150mg/kg)组及阳性对照组,共6组;采用右膝关节前交叉韧带切断术建立大鼠膝关节骨性关节炎模型,测量机械性痛阈值和膝关节直径,第14天灌胃给药,连续4周,眼球取血,脱颈椎处死,分离膝关节滑膜组织;采用ELISA法检测血清中炎症因子的分泌水平;采用RT-PCR法和Western Blot法分别检测膝关节滑膜组织中炎症因子和NF-κB通路分子的表达水平。结果:术前大鼠的机械性痛阈值和膝关节直径差异均无统计学意义(P>0.05);术后模型组大鼠的机械性痛阈值显著降低,膝关节直径显著增加,Epext显著增加KOA大鼠的机械性痛阈值和减小膝关节直径;ELISA和RT-PCR结果分别表明Epext显著抑制KOA大鼠血清和滑膜组织中IL-6,IL-1β,TNF-α的表达;苏木精-伊红染色结果表明Epext显著抑制KOA大鼠膝关节滑膜组织的炎性细胞浸润和滑膜增生;Western Blot结果表明Epext显著抑制KOA大鼠滑膜组织中p-IκB-α/IκB-α,p-p65/p65的表达,差异均有统计学意义(P<0.05)。结论:Epext通过抑制NF-κB通路对膝关节骨性关节炎治疗具有潜在价值。
Objective:To investigate the effect of total flavonoids of Epimedium(Epext)on knee osteoarthritis and related molecular mechanisms.Methods:60 male SD rats were divided into sham operation group,model group,Epext(600,300,150 mg/kg)group and positive control group for a total of 6 groups.Rats from each group were caused knee osteoarthritis(KOA)model with right knee anterior cruciate ligament severance,and the mechanical pain threshold and knee diameter were measured.On the 14 th day,the drug was administered intragastrically for 4 weeks.Blood was taken from eyeball,the cervical vertebra was sacrificed,and the knee joint synovium tissue was dissected.The secretion levels of inflammatory factors in rat serum were detected by ELISA.RT-PCR and Western Blot were used to detect the expression levels of inflammatory factors and NF-κB pathway in synovium tissue of knee joint.Results:There was no significant difference in mechanical pain threshold and knee joint diameter before operation.Compared with the sham operation group,the mechanical pain threshold of the model group was significantly decreased,and the knee joint diameter was significantly increased.Epext significantly increased the mechanical pain threshold and reduced knee diameter in KOA rats.ELISA and RT-PCR results seperately showed that Epext significantly inhibited the expression of IL-6,IL-1 and TNF-αin serum and synovium of KOA rats.HE results indicated that Epext can significantly inhibited inflammatory cell infiltration and synovium hyperplasia in the knee joint synovium tissue of KOA rats.The result of Western Blot showed that Epext ignificantly inhibited the expression of p-IκB-α/IκB-αand p-p65/p65 in the synovium tissue of KOA rats.Conclusion:Epext has potential value in the treatment of knee osteoarthritis by inhibiting the NF-κB signaling pathway.
作者
金剑飞
何维英
周钰龙
JIN Jianfei;HE Weiying;ZHOU Yulong(,Department of Orthopedics,Zhuji Hospital of Traditional Chinese Medicine,Zhuji 311800,Zhejiang China)
出处
《中国中医骨伤科杂志》
CAS
2020年第3期5-9,14,共6页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
浙江省公益技术应用研究计划项目(2016C37095).
