摘要
目的对不同基原淫羊藿药材不同部位所含的黄酮类成分进行比较,为淫羊藿质量评价及药用部位的合理选择提供依据。方法采集13批淫羊藿植株地上部分,分为叶片、叶柄、茎3个部位,对其HPLC指纹图谱和朝藿定A、B、C及淫羊藿苷、宝藿苷Ⅰ5种黄酮类成分进行分析,进而采用方差分析和中药色谱指纹图谱相似度评价软件并结合聚类分析(HCA)对淫羊藿叶片、叶柄和茎的含量差异进行综合评价。结果指纹图谱结果表明淫羊藿叶片中化学成分较茎及叶柄中丰富,叶柄与茎化学成分基本一致;含量测定结果表明淫羊藿叶片中5种成分含量均显著高于叶柄及茎,甚至达到数10倍。聚类分析结果也表明叶片与叶柄及茎可明显区分开来。结论通过分析淫羊藿不同部位化学成分差异,明确了淫羊藿叶片、叶柄及茎的质量差异,为淫羊藿质量控制及药用部位的确定提供了实验依据。
Objective To compare the flavonoids contained in different parts of different botanical origins of Epimedii Folium,and provide a basis for the quality evaluation of Epimedii Folium and the reasonable selection of medicinal parts.Methods The aerial parts of 13 batches of Epimedii Folium were collected and divided into three parts:leaf,petiole and stem.The HPLC fingerprint and content of five flavonoids,including epimedin A,epimedin B,epimedin C,icariin and baohuosideⅠ,were analyzed.Then the analysis of variance and the similarity evaluation software of traditional Chinese medicine chromatographic fingerprint were used.Combined with cluster analysis(HCA),the content differences of flavonoids in leaf,petiole and stem of Epimedii Folium were evaluated.Results The fingerprints showed that the chemical constituents in Epimedii Folium leaves were richer than stems and petioles,and the chemical constituents in petioles and stems were basically the same.The content of five components in leaves was significantly higher than that of petiole and stem,even up to 10 times.Cluster analysis also showed that the leaves were obviously distinct from the petiole and stem.Conclusion The quality differences of Epimedii Folium leaves,petioles and stems were clarified,and this study can provide the scientific evidence for the selection of medicinal parts and quality control of Epimedii Folium.
作者
许婷
黄萌萌
李瑞云
杨茹
刘丽芳
陈彦
XU Ting;HUANG Meng-meng;LI Rui-yun;YANG Ru;LIU Li-fang;CHEN Yan(Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;Multi-component of Traditional Chinese Medicine and Microecology Research Center,Jiangsu Academy of Chinese Medicine,Nanjing 210028,China;China Pharmaceutical University,Nanjing 212000,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第1期190-196,共7页
Chinese Traditional and Herbal Drugs
基金
国家重大新药创制项目(2018ZX09301030-008)
江苏省科教强卫医学重点人才项目(ZDRCA2016036).
