摘要
目的检测老年性白内障进展中GATA4表达量的变化。方法选取老年性白内障晶状体前囊膜32例作为实验组,选取年龄相匹配的玻璃体切割术中撕除的无明显白内障的晶状体囊膜28例作为对照组。所有样本应用qRT-PCR技术及全自动蛋白印迹定量分析系统WES检测GATA4及mRNA的表达量。结果应用qRT-PCR技术及全自动蛋白印迹定量分析系统WES检测发现实验组晶状体前囊膜中的GATA4的蛋白表达量高于对照组(平均为4.19倍,P=0.029),GATA4的mRNA表达量亦显著高于对照组(平均为2.15倍,P=0.039)。结论老年性白内障的发展过程中,GATA4的蛋白表达量及GATA4的m RNA表达水平均明显增高,考虑伴随GATA4表达的提高,促进了老年性白内障的发生发展,其与老年性白内障形成有关。
Objective To detect the change of GATA4 expression in the progression of senile cataract.Methods Lens anterior capsule of senile cataract among 32 cases were selected as the experimental group,and 28 cases of lens capsule without obvious cataract removed during age-matched vitrectomy were selected as the control group.All samples were analyzed by qRT-PCR technology and automatic western blot quantitative analysis WES system to detect the expression of GATA4 and mRNA.Results The results showed that the protein expression of GATA4 in the lens anterior capsule of the experimental group was higher than that of the control group(mean4.19 times,P=0.029),and GATA4 mRNA expression was also significantly higher than the control group(average 2.15 times,P=0.039).Conclusion During the development of senile cataract,the expression of GATA4 protein and the level of GATA4 mRNA were significantly increased.It is considered that the increase of GATA4 expression promoted the occurrence and development of senile cataract.It is related to the formation of senile cataract.
作者
古丽努尔·买买提
张文娟
谢小东
Gurinur Maimaiti;ZHANG Wenjuan;XIE Xiaodong(People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi,830001,China)
出处
《新疆医学》
2021年第3期317-319,共3页
Xinjiang Medical Journal
基金
新疆维吾尔自治区自然科学基金面上项目(项目编号:2017D01C118)
