摘要
目的优化石杉碱甲二元醇质体的处方,并对其理化性质进行考察。方法采用注入法制备石杉碱甲二元醇质体。以卵磷脂含量(X_(1))、无水乙醇含量(X_(2))、混合醇含量(X_(3))为考察因素,以包封率(Y_(1))与平均粒径(Y_(2))为评价指标,通过星点设计-响应面法优化醇质体处方。并考察醇质体的粒径、电位等理化性质。结果优选的处方为卵磷脂含量3.60%,无水乙醇含量22.50%,混合醇含量45.00%,在此条件下,石杉碱甲二元醇质体的平均包封率为(94.05±0.22)%,平均粒径为(260.1±5.7)nm,Zeta电位为(-20.9±0.3)mV,PDI值为0.188±0.056。结论星点设计-响应面法建立的响应方程具有较好的预测性,可用于石杉碱二元醇质体处方的优化,所得二元醇质体性质稳定。
OBJECTIVE To optimize the formulation of huperzine A binary ethosomes and investigate its physicochemical properties.METHODS The injection method was used to prepare huperzine A binary ethosomes.Taking lecithin content(X_(1)),absolute ethanol content(X_(2))and mixed alcohol content(X_(3))as the inspection factors,central composite design-response surface method and overall desirability were used to optimize the formulation with the encapsulation efficiency(Y_(1))and average particle size(Y_(2))as evaluation indicators.The physicochemical properties of ethosomes such as particle size and potential were investigated.RESULTS The optimum preparation of formulation were the lecithin content of 3.60%,the absolute ethanol content of 22.50%and the mixed alcohol content of 45.00%.Under these conditions,the average encapsulation efficiency was(94.05±0.22)%,the average particle size was(260.1±5.7)nm,the Zeta potential was(-20.9±0.3)mV and the PDI was 0.188±0.056.CONCLUSION The response equation established by central composite design-response surface method has good predictability,and can be used to optimize the formulation of huperzine A binary ethosomes.The obtained ethosomes have stable properties.
作者
冯连晶
陈筱瑜
黄庆德
陈丹
FENG Lianjing;CHEN Xiaoyu;HUANG Qingde;CHEN Dan(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Department of Pharmacy,Fujian Health College,Fuzhou 350101,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2022年第2期181-186,共6页
Chinese Journal of Modern Applied Pharmacy
基金
福建省科技厅引导性项目(2018Y0050)
关键词
石杉碱甲
二元醇质体
处方优化
星点设计-响应面法
huperzine A
binary ehosomes
prescription optimization
central composite design-response surface method
