摘要
在添加外源耐盐石油烃降解菌Staphylococcus 2-24的基础上,研究了1.0%、2.0%、3.0%NaCl与土壤中石油烃降解率、对应微生物数量、微生物菌群结构及优势菌变化的关系。结果显示,特定的菌群在高盐中(3.0%NaCl)的定殖能力强于低盐环境(1%NaCl);石油烃的降解率、可培养微生物总数及石油烃降解菌的数量与盐度呈负相关;菌群丰度及多样性在修复前期与盐度相关性较小,修复后期与盐度正相关;盐胁迫显著影响了菌群结构及优势属的相对丰度,盐胁迫下优势属为KCM-B-112、Halomonas、Virgibacillus,菌群结构及优势属随修复时间的推移而变化。
The corresponding relationship between the concentrations(1%,2%and 3%)of NaCl in the soil respectively and the degrading rate of petroleum hydrocarbons in the soil,corresponding microbial quantity,microbial flora structure and dominant bacteria change are studied on the basis of adding with salt-tolerant degrading strain Staphylococcus2-24 during the remediation process.The result shows that a specific flora represents a stronger colonization ability in high salt environment(3.0%NaCl)than that in low salt environment(1%NaCl).The degrading rate of petroleum hydrocarbons,total number of culturable microorganisms and the number of degrading bacteria for petroleum hydrocarbon are all negative correlation with NaCl concentration.The abundance and diversity of microflora have little correlation with NaCl concentration in the early stage of remediation,and have a positive correlation with NaCl concentration in the late stage of restoration.Salt stress affects significantly the structure of microflora and the relative abundance of dominant bacteria.KCM-B-112,Halomonas and Virgibacillus are the dominant bacteria under salt stress.Salinity changes the bacterial community structure under the experimental conditions,and both the flora structure and the dominant bacteria change with the degradation process of petroleum hydrocarbons in the soil.
作者
侯心然
李琪
李天元
李田
孙小喜
祁志冲
张强
HOU Xin-ran;LI Qi;LI Tian-yuan;LI Tian;SUN Xiao-xi;QI Zhi-chong;ZHANG Qiang(Shandong Province Key Laboratory of Applied Microbiology,Ecology Institute of Shandong Academy of Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China;Key Laboratory of Pollution Processes and Environmental Criteria of the Ministry of Education,College of Environmental Science and Engineering,Nankai University,Tianjin 300350,China;Gudao Oil Production Plant,Sinopec Shengli Oilfield Branch Company,Dongying 257231,China;International Joint Laboratory for Environmental Pollution Control Materials of Henan Province,College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,China)
出处
《现代化工》
CAS
CSCD
北大核心
2022年第S02期98-102,107,共6页
Modern Chemical Industry
基金
NSFC-山东联合基金(U1906222)
国家重点研发计划(2019YFC1804104)
国家自然科学基金(41807111)
关键词
石油污染
土壤修复
生物强化
盐胁迫
菌群结构
petroleum polluted
soil remediation
bio-enhanced
salt stress
flora structure
