摘要
为了探究滨海湿地土壤微生物和土壤酶活性对氮输入和盐渍化的生态响应,在天津滨海湿地采集土壤,进行不同氮输入水平和盐度的交互实验.氮输入水平分别为0、20和40 g/(m^2·a),盐度分别为0.1%和1.0%,研究不同处理下土壤可培养细菌和真菌的群落组成、数量、群落多样性和土壤酶活性.从天津滨海湿地土壤中分离到可培养细菌和真菌各10个属:在不同氮输入水平和盐度处理组合中均有分布的细菌有农杆菌属、微杆菌属、溶杆菌属和芽孢杆菌属,真菌有曲霉属、拟青霉属、镰刀菌属、青霉属和多环孢霉属.总体上,随着氮输入水平的增加,土壤细菌数量增加,而真菌数量降低,且细菌与真菌群落的多样性均呈降低趋势.随土壤盐度的增加,土壤细菌和真菌数量及多样性均有所降低.氮输入的增加显著促进了土壤蔗糖酶、β-葡萄糖苷酶和脲酶的活性,而土壤蛋白酶活性随氮输入增加先升高再降低;盐度增加对土壤酶活性没有显著影响.
To explore the ecological responses of soil microbial communities to nitrogen input and salinization in coastal wetlands,the soil samples were collected from Tianjin coastal wetland.The soil culturable bacterial and fungal community compositions,quantities,diversity and soil enzyme activities were studied using a full factorial experiment with different nitrogen input treatments,i.e.0,20 and 40 g/(m^2·a),and different salinity treatments,i.e.0.1%and 1.0%.Ten genuses of soil culturable bacteria and fungi were found respectively.The bacterial genuses found in all treatments included Agrobacterium,Microbacterium,Lysobacter,Bacillus;the fungal genuses found in all treatments included Aspergillus,Paecilomyces,Fusarium,Penicillium,Plectosphaerella.In general,with the increase of nitrogen input level,the quantity of soil bacteria increased,while the quantity of fungi decreased,and the diversities of soil bacteria and fungi both decreased.With the increase of salinity,the quantities and diversities of soil bacteria and fungi both decreased.The increase of nitroen input level promoted the activities of soil sucrase,β-glucosidase and urease,while the activity of soil protease increased at first and then decreased with the increase of nitrogen input level.Salinity did not have significant effects on soil enzyme activities.
作者
王春萍
王银华
霍玉珠
侯涛亮
马成仓
郭宏宇
WANG Chunping;WANG Yinhua;HUO Yuzhu;HOU Taoliang;MA Chengcang;GUO Hongyu(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)
出处
《天津师范大学学报（自然科学版）》
CAS
北大核心
2020年第1期40-47,共8页
Journal of Tianjin Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31300357)
天津市自然科学基金资助项目(16JCYBJC44100)
天津市教委科研计划项目(2018KJ156)
天津师范大学引进人才基金资助项目(5RL140,5RL149).
关键词
氮输入
盐渍化
滨海湿地
土壤微生物群落
土壤酶活性
nitrogen input
salinization
coastal wetland
soil microbial community
soil enzyme activity
