摘要
目的该文对复方槐花散总黄酮提取工艺及总黄酮的抗氧化活性进行研究。方法利用紫外-可见分光光度法在352nm处测定其吸光度并计算其含量,以VC为对照,评价槐花散总黄酮的抗氧化活性。结果槐花散总黄酮最佳提取工艺:液料比30∶1(mL∶g),甲醇浓度90%,回流提取70 min,温度80 C,复方槐花散总黄酮提取率为18.98 mg/g。槐花散总黄酮对DPPH自由基有显著的清除力,当浓度为35μg/mL时,对DPPH自由基的清除率为95.20%,其抗氧化活性优于VC。结论甲醇回流提取法切实可行,所提取的总黄酮抗氧化活性较好,可用于槐花散总黄酮的提取。
Objective In this paper,the extraction technology and the antioxidant activity of total flavonoids were studied from Huaihua Powders.Methods The content was calculated by measuring the absorbance at 352 nm using ultraviolet-visible method.The antioxidant activity of total flavonoids in Huaihua Powders was evaluated by using Vc as a control.Results The optimum extraction technology of flavonoids from Huaihua Powders was as follow:30:1 liquid/solid ratio,90%methanol concentration,reflux extraction 70 min at 80°C.The extraction rate of total flavonoids was 18.98 mg/g.The total flavonoids in Huaihua Powders had obvious scavenging effect on DPPH free radical.The clearance rate of DPPH free radical was 95.20%when the concentration was 35μg·mL-1.The antioxidant activity of total flavonoids was superior compared to VC.Conclusion Methanol reflux extraction was feasible and the obtained total flavonoids had excellent antioxidant activity.It could be used to extract flavonoids from Huaihua Powders.
作者
陈星
罗晓
史艳梅
雷敬卫
陈志红
CHEN Xing;LUO Xiao;SHI Yan-mei;LEI Jing-wei;CHEN Zhi-hong(First Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou Henan 450000 China;Henan University of Chinese Medicine,Zhengzhou Henan 450046 China;Henan Province Traditional Chinese Medicine Quality Control and Evaluation Engineering Technology Research Center,Zhengzhou Henan 450046 China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2020年第10期2363-2365,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家重点研发计划“中医药现代化研究”重点专项(2018YFC1707000)
关键词
槐花散
总黄酮
提取工艺
含量测定
抗氧化活性
Huaihua Powders
Total flavonoids
Extraction process
Content determination
Antioxidant activity
