摘要
目的建立了一种同时测定大豆苷元及其三个氨基甲酸酯前药的含量的高效液相色谱方法。方法选用Welch Ultimate LP-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A),体积分数0.3%甲酸水(B),甲醇(C)进行三元梯度洗脱,流速1.0 mL·min^-1,检测波长249 nm,柱温30℃。结果应用所建立的方法,各组分在24 min内均得到较好分离。大豆苷元(Dan)、7-缬氨酸大豆苷元氨基甲酸酯(7-P)、4′-缬氨酸大豆苷元氨基甲酸酯(4′-P)和7-4′-二缬氨酸大豆苷元氨基甲酸酯(7-4′-P)的质量浓度分别在1.003~20.06 mg·L^-1、1.004~20.08 mg·L^-1、1.002~20.04 mg·L^-1、1.001~20.02 mg·L^-1范围内与峰面积呈良好的线性相关性,平均回收率分别为100.1%、100.5%、100.1%、100.2%(n=9),RSD均小于2.0%。结论所建立的方法可用于大豆苷元及其氨基甲酸酯前药的含量测定及其化学稳定性研究。
Objective To develop ahigh performance liquid chromatography method for the simultaneous determination of the content of daidzein and its three carbamate prodrugs(7-P,4′-P and 7-4′-P).Methods The mobile phase was consisted of acetonitrile-methanol-0.3%formic acid of water solution(V∶V)with a ternarygradient program.The separation was performed by Welch Ultimate LP-C18(250 mm×4.6 mm,5μm)at the flow rate of 1.0 mL·min^-1 and the test was monitored at the wavelength of 249 nm.Results The blank solvent had no interference to the determination.Daidzein,7-P,4′-P and 7-4′-P could be effectively separated with the resolution greater than 1.5.In addition,daidzein,7-P,4′-P and 7-4′-P had good linear relationships with the peak area of 1.003-20.06μg·mL^-1,1.004-20.08 mg·L^-1,1.002-20.04 mg·L^-1 and 1.001-20.02 mg·L^-1,respectively.The average recoveries of daidzein,7-P,4′-P and 7-4′-P were 100.1%(RSD=0.60%),100.5%(RSD=0.35%),100.1%(RSD=0.57%),100.2%(RSD=0.45%).Conclusion The developed method can be used for the simultaneous determination and chemical stability of daidzein and its three carbamate prodrugs.
作者
白晓晨
李颖超
张天虹
侯晓虹
BAI Xiaochen;LI Yingchao;ZHANG Tianhong;HOU Xiaohong(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;Wuya College of Innovation,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2019年第12期1093-1099,共7页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(81573497).
关键词
大豆苷元
前药
高效液相色谱法
含量测定
daidzein
prodrug
high performance liquid chromatography
content determination
