摘要
比较全蝎白术白头翁组合发酵品(SAPZFP)与未发酵品(SAPZP)水提液不同截留分子量冻干粉活性成分及体外抗肿瘤活性的差异。分别测定各截留分子量段水溶性氨基酸、蛋白质、多糖的含量,并采用MTT法研究其对乳腺癌细胞株MCF-7的抑制作用。SAPZFP截留分子量<5、5~10、>10 kDa冻干粉对乳腺癌细胞株MCF-7的IC50分别为0.854、0.315、0.071 mg·mL-1;SAPZP的分别为1.128、0.360、0.115 mg·mL-1。SAPZFP截留分子量>10kDa的抗肿瘤活性显著,球孢白僵菌双向固体发酵技术可显著增强本组合物的体外抗肿瘤活性。
The active constituents and anti-tumor activity in vitro among different molecular weight range of lyophilized powders of SAPZFP’s and SAPZP’s water extract were compared.The contents of water-soluble amino acid,protein and polysaccharide in various molecular weight ranges were determined and the inhibitory effect of the drugs on mammary cancer cells strain MCF-7 was studied by MTT assay.The IC50 of SAPZFP with<5,5~10,>10 kDa on mammary cancer cells strain MCF-7 were 0.854,0.315,0.071 mg·mL-1,respectively.The IC50 of SAPZP were 1.128,0.360,0.115 mg·mL-1,respectively.The anti-tumor activity of SAPZFP with>10 kDa was significant,and the bidirectional solid fermentation technology of Beauveria bassiana can markedly enhance the anti-tumor activity in vitro.
作者
刘金虎
赵国庆
毛会秀
方园
陶然
张瑞
史磊
王集会
LIU Jin-hu;ZHAO Guo-qing;MAO Hui-xiu;FANG Yuan;TAO Ran;ZHANG Rui;SHI Lei;WANG Ji-hui(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Shandong Jinan 250355,China;Center of Experiment,Shandong University of Traditional Chinese Medicine,Shandong Jinan 250355,China)
出处
《当代化工》
CAS
2020年第1期32-36,共5页
Contemporary Chemical Industry
基金
山东省重点研发计划项目,项目号:2016GSF202003
山东省高等学校科技计划项目,项目号:J17KA244
山东省中医药科技发展计划项目,项目号:2017-025
山东中医药大学大学生研究训练(SRT)计划项目,项目号:2018042.
关键词
中药组合品
超滤
水溶性活性成分
抗肿瘤
Chinese medicine composition
Ultrafiltration
Water-soluble active constituents
Anti-tumor
