摘要
目的通过分析南阳艾不同部位内生菌菌群结构多样性及其与关键次生代谢产物含量之间的关系,探究南阳艾内生菌对其药材品质的影响。方法本研究采用高通量测序技术分析南阳艾不同组织内生菌群落的多样性和组成;利用高效液相色谱技术检测南阳艾不同组织中关键代谢产物的含量;进而通过皮尔逊相关性分析,筛选与南阳艾关键次生代谢产物显著相关的内生菌群落。结果南阳艾内生细菌和真菌多样性和组成具有显著组织差异,根中细菌和真菌多样性指数(Chao和Ace)均显著高于茎和叶。通过LEfSe分析,确定根瘤菌属、链霉菌属、黄单胞菌科、子囊菌纲等为南阳艾生物标记物。相关性分析的结果表明,根瘤菌属、假单胞菌属、黄杆菌属、链霉菌属、曲霉属、油壶菌属等属的丰度与部分苯丙素类和黄酮类代谢物的含量呈现显著正相关(P<0.05)。结论本研究筛选了一些南阳艾次生代谢物显著相关的内生菌群落,为进一步分离南阳艾关键次生代谢产物相关的内生菌株,提升南阳艾的品质提供了科学依据。
Objective To explore the effect of endophytes on Nanyang Mugwort by analyzing the diversity of endophytic flora and its correlation with the content of key secondary metabolites in three compartments of Nanyang Mugwort,and the effect of medicinal material quality.Methods In this study,high-throughput sequencing and high-performance liquid chromatography(HPLC)were carried out to determine the distribution endophytes and key secondary metabolites in three compartments of Nanyang Mugwort respectively.The endophytic communities that were significantly correlated with key secondary metabolites were screened by Pearson correlation analysis.Results We found endophytic diversity and composition showed compartment specificity,and bacterial and fungal alpha diversity values(Chao and Ace)in the root compartment were significantly higher than that of leaf and stem.Linear discriminant analysis efect size(LEfSe)analyses identified some potential microbial biomarkers,such as Rhizobium,Streptomyce,Xanthomonadaceae,and Sordariomycetes.Pearson correlation analysis showed that endophytes(such as Rhizobium,Pseudomonas,Flavobacterium,Streptomyces Aspergillus,and Olpidium)were significantly correlated with contents of some phenylpropanoid and flavonoid metabolites in our study(P<0.05).Conclusion In this study,some endophytic communities with significant correlation between key secondary metabolites of Nanyang Mugwort were identified,which provided valuable information to guide the isolation of endophytic strains related to key secondary metabolites and improve the quality of Nanyang Mugwort.
作者
李孟芝
李超
崔占虎
韦艳
朱琳
黄显章
LI Mengzhi;LI Chao;CUI Zhanhu;WEI Yan;ZHU Lin;HUANG Xianzhang(Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Zhang Zhong-jing College of Traditional Chinese Medicine,Nanyang Institute of Technology,Nanyang 473004,China;The First People's Hospital of Nanyang Affiliated to Henan University,Nanyang 473010,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2024年第5期1202-1212,共11页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家财政部和农业农村部国家现代农业产业技术体系项目(CARS-21):山药焦作综合试验站(国家中药材产业技术体系艾叶单品种攻关小组),负责人:黄显章
南阳理工学院博士科研启动基金(NGBJ-2022-03):艾草内生菌多样性及其促进宿主活性代谢物积累的可利用菌株筛选,负责人:李孟芝
国家自然科学基金委员会青年科学基金(82204565):基于艾叶品质形成过程的腺毛发育与黄酮合成共调控基因挖掘与鉴定,负责人:崔占虎
关键词
南阳艾
内生菌
多样性
组成
次生代谢产物
Nanyang Mugwort
Endophytes
Diversity
Composition
Secondary metabolites
