摘要
目的建立以对照药材为基准物质定性和不依赖多种对照品定量的荆芥穗药材“质-量”分析方法。方法采用HPLC技术,以对照药材为基准物质建立荆芥穗特征图谱,通过Q-TOF-MS技术,鉴定共有峰的化学成分,以对照药材和供试药材特征峰的相似度,明确药材真伪,即“质”;对内标成分“胡薄荷酮”进行准确定量,以内标成分计,计算不同批次供试品中各特征峰的相对含量,取“平均数-标准差”作为特征峰化学成分相对于内标物质化学成分的相对含量下限,依据特征峰相对含量下限明确荆芥穗药材优劣,即“量”。采用HPLC技术建立荆芥穗对照药材的特征图谱,将其与供试药材图谱进行比对来辨别药材的真伪,即“质”;采用内标物质对特征峰化学成分进行相对定量来区分药材的优劣,即“量”。结果建立的特征图谱及含量测定方法符合方法学考察要求;确定了11个共有色谱峰,鉴定出了6个化学成分,分别为木犀草苷、橙皮苷、木犀草素-7-O-葡萄糖醛酸苷、木犀草素、香叶木素、胡薄荷酮,各荆芥穗供试药材与荆芥穗对照药材的相似度均大于0.90;规定了荆芥穗药材特征峰化学成分相对含量下限。结论该方法不依赖多种对照品,能清晰、快速地判断药材的真伪优劣,为荆芥穗的质量控制方法提供参考。
Objective To establish a"quality-quantity"double standard control method of Schizonepeta Spike based on the reference material without relying on multiple reference substances.Methods HPLC technology was used to establish the characteristic atlas of Schizonepeta Spike based on the reference traditional Chinese medicinal materials.The chemical constituents of common peaks were identified by Q-TOF-MS technology,and the authenticity of Schizonepeta Spike was determined by the similarity of characteristic peaks of the reference traditional Chinese medicinal materials and the tested traditional Chinese medicinal materials.The relative content of each characteristic peak in different batches of the tested traditional Chinese medicinal materials was calculated by accurate quantition of pulegone.The"Average value-Standard deviation"was taken as the lower limit of the relative content of characteristic peak chemical components relative to pulegone,and the quality of Schizonepeta Spike was determined according to the lower limit of the relative content of characteristic peak.Results The characteristic atlas and content determination method met the requirements of methodology investigation.A total of 11 common chromatographic peaks were determined,and 6 chemical components were identified,which were luteolin,hesperidin,luteolin-7-O-glucuronide,luteolin,geranyl,pulegone.The similarity between the tested traditional Chinese medicinal materials and reference traditional Chinese medicinal materials was greater than 0.90.The lower limit of relative content of chemical components of Schizonepeta Spike characteristic peak was defined.Conclusion The method can identify Schizonepeta Spike clearly and quickly without many reference substances,and provide reference for quality control of Schizonepeta Spike.
作者
贾梦楠
李天娇
包永睿
王帅
孟宪生
Jia Mengnan;Li Tianjiao;Bao Yongrui;Wang Shuai;Meng Xiansheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Multi-Dimensional Analysis of Traditional Chinese Medicine Technical Innovation Center,Dalian 116600,China;Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory,Dalian 116600,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第11期3506-3514,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
辽宁省科学技术厅社发攻关及产业化(2020JH2/10300088):基于中医典籍的经典名方槐花散药学研究,负责人:孟宪生
关键词
荆芥穗
对照药材
质量控制
特征图谱
胡薄荷酮
Schizonepeta Spike
Reference crude drug
Quality control
Specific chromatogram
Pulegone
