摘要
目的为了探究补土雅解方对抗生素相关性腹泻大鼠的肠道屏障调节作用。方法60只SD雄性大鼠,随机分为空白组、模型组、阳性药组(1 g·kg^(-1)),补土雅解方高、中、低剂量组(40.5、20.25、10.125 g·kg^(-1))。采用盐酸林可霉素(5 g·kg^(-1))灌胃法复制模型,连续7天。造模成功后,进行药物干预7天后取材。HE染色观察肠道病理形态;ELISA试剂盒检测DAO、MPO、LPS;取各脏器组织检测细菌移位;收集粪便进行16S rRNA基因高通量测序分析。结果与正常组相比,模型组大鼠血清中DAO、MPO、LPS含量显著提高(P<0.001、P<0.01),肠道黏膜上sIgA含量明显下降(P<0.001),促进肠道细菌移位(P<0.001、P<0.01)。肠道菌群多样性明显降低,肠道微生物门、属水平均发生明显变化。补土雅解方可降低DAO、MPO、LPS含量(P<0.001、P<0.01、P<0.05),显著提高sIgA含量(P<0.01、P<0.05),有效抑制肠道细菌移位(P<0.001、P<0.01、P<0.05)。同时其通过升高厚壁菌门,抑制拟杆菌门和变形菌门比例,以及调控乳酸菌属、鞘氨醇单胞菌属、绿脓杆菌属、肠杆菌属来纠正肠道微生态结构。结论补土雅解方能够降低肠道黏膜通透性、减少肠道菌群移位、保护肠道免疫屏障、调节肠道菌群结构的多样性、改善肠道微生态紊乱,可有效治疗抗生素相关性腹泻。
Objective To explore the intestinal barrier regulation effect of Butuyajie recipe on antibiotic-associated diarrhea rats.Methods 60 SD male rats were randomly divided into blank group,model group,positive drug group(1 g·kg^(-1)),and high-dose,medium-dose and low-dose groups of Butuyajie recipe(40.5,20.25,10.125 g·kg^(-1)).The model was replicated by intragastric administration of lincomycin hydrochloride(5 g·kg^(-1))for 7 consecutive days.After successful modeling,the materials were obtained after drug intervention for 7 days.Intestinal pathological morphology was observed by HE staining.ELISA kit to detect DAO,MPO,LPS.Take each organ tissue to detect bacterial translocation.Feces were collected for 16S rRNA gene high-throughput sequencing analysis.Results Compared with the normal group,the serum levels of DAO,MPO and LPS in the model group were significantly increased(P<0.001,P<0.01),and the sIgA content in the intestinal mucosa was significantly decreased(P<0.001).Promote intestinal bacterial translocation(P<0.001,P<0.01).The diversity of intestinal flora was significantly reduced,and the levels of intestinal microflora and genera were significantly changed.Butuyajie recipe can reduce the content of DAO,MPO and LPS(P<0.001,P<0.01,P<0.05),significantly increase the content of sIgA(P<0.01,P<0.05),and effectively inhibit the translocation of intestinal bacteria(P<0.001,P<0.01,P<0.05).At the same time,it corrects the intestinal microecological structure by increasing Firmicutes,inhibiting the proportion of Bacteroidetes and Proteobacteria,and regulating Lactobacillus,Sphingomonas,Pseudomonas,and Enterobacter.Conclusion Butuyajie recipe can reduce the permeability of intestinal mucosa,reduce the translocation of intestinal flora,protect the intestinal immune barrier,regulate the diversity of intestinal flora structure,improve the intestinal microecological disorder,and can effectively treat antibiotic-associated diarrhea.
作者
肖湉
于兴志
杨丽萍
段小花
Xiao Tian;Yu Xingzhi;Yang Liping;Duan Xiaohua(Yunnan Key Laboratory of Dai and Yi Medicines,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第8期2743-2751,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
云南省傣医药与彝医药重点实验室自由探索课题项目(2020Z002):基于肠道微生物研究补土雅解方解药物毒的作用机制,负责人:段小花
中共云南省委人才工作领导小组办公室人才项目(XDYC-QNRC-2022-0284):云南省兴滇英才支持计划“青年人才”,负责人:段小花。
