摘要
目的基于“肠-脑”相关理论观察针刺对偏头痛模型大鼠肠道菌群和中枢炎症的影响,以阐明针刺治疗偏头痛的“肠-脑”作用机制。方法采用硝酸甘油皮下注射法制作偏头痛大鼠模型,并随机分为模型组和针刺组,每组6只,另设空白对照组6只进行常规捆绑固定。在造模前及开始造模后第1、5、9天各组均采用VonFrey测定大鼠足底机械痛阈。实验结束后应用ELISA检测各组大鼠中枢三叉神经脊束核炎性因子白介素-6(Interleukin-6,IL-6)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的表达含量。采用三代Pacbio全长微生物多样性测序对各组粪便样本进行16S全长rDNA测序,检测各组样本间操作分类单元(Operational taxonomic units,OTU)聚类及其丰度、Alpha多样性指数、Beta多样性指数、物种丰度等方面的差异。结果偏头痛模型大鼠存在足底机械痛阈显著降低(P<0.01)、中枢IL-6、TNF-α的含量显著升高(P<0.01)、肠道菌群的结构和丰度发生异常改变(P<0.01);持续针刺治疗可显著升高偏头痛大鼠的足底机械痛阈(P<0.01),调节偏头痛大鼠肠道菌群多样性,并升高鼠乳杆菌、降低肠乳杆菌菌群丰度(P<0.05),降低偏头痛模型大鼠中枢IL-6、TNF-α的含量(P<0.01)。结论针刺可能通过调节偏头痛大鼠肠道菌群结构和中枢IL-6、TNF-α炎症因子的表达,发挥针刺治疗偏头痛的“肠-脑”抗炎镇痛作用。
Objective Based on the theory of"gut-brain",this study explored the effect of acupuncture on the gut microbiota and central inflammation in migraine model rats,in order to explore the mechanism of acupuncture in the treatment of migraine from the perspective of"gut-brain".Methods The migraine rat model was established by subcutaneous injection of nitroglycerin.They were randomly divided into a model group and an acupuncture group,with 6 rats in each group,and a control group with 6 rats for conventional binding and fixation.Before modeling and on the 1st,5th,and 9th days after modeling,each group used electronic VonFrey to measure the plantar mechanical pain threshold of rats.After the experiment,Elisa was used to detect the expression levels of inflammatory factors IL-6 and TNF-αin the central trigeminal spinal nucleus of the rats in each group.Three-generation Pacbio full-length microbial diversity sequencing was used to perform 16S full-length rDNA sequencing on each group of fecal samples to detect the operational taxonomic unit(OTU)clustering and its abundance,Alpha diversity index,Beta diversity index,species among the samples in each group.differences in abundance.Results In migraine model rats,plantar mechanical pain threshold was significantly decreased(P<0.01),central IL-6 and TNF-αcontents were significantly increased(P<0.01),and the structure and abundance of gut microbiota were abnormal.change(P<0.01).Continuous acupuncture treatment can significantly increase the plantar mechanical pain threshold in migraine rats(P<0.01),regulate the diversity of gut microbiota in migraine rats,increase Lactobacillus murine,and reduce the abundance of Lactobacillus enterobacteriaceae.degree(P<0.05),and decreased the levels of IL-6 and TNF-αin the central nervous system of migraine model rats(P<0.01).Conclusion Acupuncture can exert the"gut-brain"anti-inflammatory and analgesic effect of acupuncture in the treatment of migraine by regulating the gut microbiota structure and the expression of central IL-6 and TNF-αinflammatory factors in migraine model rats.
作者
孙铭声
陈星宇
周梦荻
刘一
郑宇琦
黄思莹
蔡定均
赵凌
Sun Mingsheng;Chen Xingyu;Zhou Mengdi;Liu Yi;Zheng Yuqi;Huang Siying;Cai Dingjun;Zhao Ling(College of Acupuncture and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Chengdu Hospital of Integrated Traditional Chinese and Western Medicine,Chengdu 610041,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第8期2726-2734,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家科学技术部重点研发计划“中医药现代化研究”重点专项课题(2019YFC1709701):“宣阳解郁,通络止痛”针法治疗偏头痛的循证评价及转化研究,负责人:赵凌
国家自然科学基金青年科学基金项目(82004486):基于肠道菌群介导SCFAs调控小胶质细胞活化的循经取穴针刺治疗偏头痛效应机制研究,负责人:孙铭声
中国博士后科学基金面上项目(2020M683643XB):基于肠道菌群介导针刺治疗慢性偏头痛的镇痛机制研究,负责人:孙铭声。
关键词
偏头痛
针刺
肠道菌群
中枢炎症
三代Pacbio全长微生物多样性测序
Migraine
Acupuncture
Gut microbiota
Central inflammation
Full-length microbial diversity sequencing of the third-generation Pacbio
