摘要
目的优化《中华人民共和国药典》2015年版山楂叶提取物工艺的分离纯化条件,提高山楂叶提取物制备的达标率。在现有的针对总黄酮研究的基础上,提高牡荆素鼠李糖苷的含量,符合山楂叶提取物用药要求。方法以牡荆素鼠李糖苷含量为指标,考察了山楂叶提取参数、分离纯化过程中的树脂型号、洗脱体系等。结果选用LX158树脂替代药典规定的D101树脂,采用3BV水洗除杂后,3BV 10%乙醇洗脱除杂,洗脱剂为6BV 30%乙醇。结论优化后的工艺总黄酮含量≥80%,牡荆素鼠李糖苷含量≥8.8%,满足药典相关要求且较比药典工艺收率高7.07%,为中国药典山楂叶提取物制备工艺的补充和修订提供科学依据。
Objective To optimize the separation and purification conditions of Hawthorn Leaf Extract in Chinese Pharmacopoeia 2015 edition,and to improve the standard rate of preparation of Hawthorn Leaf Extract.On the basis of existing research on total flavonoids,the content of Vitexin rhamnoside was increased,which met the medication requirements of hawthorn leaf extract.Methods With the content of Vitexin rhamnoside as the index,the extraction parameters,resin type and elution system of hawthorn leaves were investigated.Result LX 158 resin was used to replace D101 resin prescribed in Pharmacopoeia.After washing impurities with 3 BV water,3 BV 10%ethanol was used to remove impurities.The eluent was 6 BV 30%ethanol.Conclusion The content of total flavonoids and rhamnoside in the optimized process is more than 80%and 8.8%respectively,which meet the requirements of Pharmacopoeia and the yield is 7.07%higher than that of the Pharmacopoeia process.It provides a scientific basis for the supplement and revision of the preparation process of hawthorn leaf extract in Chinese pharmacopoeia.
作者
姜红红
王红霞
连运河
乔延江
高伟
Jiang Honghong;Wang Hongxia;Lian Yunhe;Qiao Yanjiang;Gao Wei(School of Chinese Pharmacy Beijing University of Chinese Medicine,Beijing 102488,China;Chenguang Biotech Group Company Limited,Research and Development Center Nutrition Extract Laboratory,Handan 057250,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第8期2883-2892,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
河北省晨光生物科技集团股份有限公司研发部建设专项(18967136H)
河北省植物天然色素产业技术研究院绩效评估后补助经费,负责人:连运河
关键词
山楂叶提取物
总黄酮
牡荆素鼠李糖苷
工艺优化
Hawthorn leaf extract
Total flavonoid
Vitexin rhamnoside
Process optimization
