摘要
目的采用简单序列重复区间(ISSR)分子标记技术分析不同产地温莪术的遗传多样性和亲缘关系。方法以3个产地8个温莪术居群的42个样品为材料,从20条ISSR引物中筛选出6条进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测后,利用PopGene 32软件对不同居群ISSR标记结果进行多态性分析,通过UPGMA方法聚类并构建亲缘关系树。结果6条引物共扩增出42个多态性位点,居群水平多态位点平均百分率为29.17%。居群间遗传分化系数Gst为0.3886,居群间每代个体的基因流系数(Nm)为0.7868。8个居群遗传一致性范围为0.8481-0.9736,遗传距离范围为0.0268-0.1647。结论供试8个温莪术居群之间的遗传多样性水平偏低,遗传变异较少,基因流动性水平较低。本研究的开展为温莪术的引种栽培及育种提供了一定的分子生物学依据。
Objective To analyze the genetic diversity and phylogenetic relationship of Curcuma wenyujin Y.H.Chen et C.Ling from different habitats with ISSR markers.Methods Totally 42 samples from 8 populations of 3 habitats were collected as materials.6 primers screened from 20 ISSR primers were used for PCR amplification and the amplification products were detected by agarose gel electrophoresis.Popgene32 software was used for polymorphic analysis of different populations and UPGMA method was applied for cluster analysis and constmction of phylogenetic tree.Results Totally42 polymorphic loci were obtained from 6 primers.Percentage of polymorphic loci at population level was 29.17%.The coefficient of gene differentiation(Gst)among populations was 0.3886 and gene flow coefficient(Nm)of individuals was0.7868.Genetic similarity coefficient of 8 populations was 0.8481-0.9736 and the genetic distances was 0.0268-0.1647.Conclusion Genetic diversity of C.wenyujin from different populations is relatively low.Difference of individuals from different habitats is small at molecular level.The present study provides molecular evidence for introduction,cultivation and breeding of C.wenyujin.
作者
张玉秀
刘杨
刘培卫
留诗雨
黄娴
魏建和
Zhang Yuxiu;Liu Yang;Liu Peiwei;Liu Shiyu;Huang Xian;Wei Jianhe(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Hainan Bikai Institute of Materia Medica Co.,Ltd.Haikou 570216,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第8期2845-2850,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
海南省科学技术厅重大科技研发专项(ZDKJ2016006):莪术新药研发及现有中成药大品种保妇康栓的技术升级研究,负责人:史朝晖
关键词
温莪术
简单序列重复区间
遗传多样性
亲缘关系
Curcuma wenyujin
Inter-simple sequence repeat
Genetic diversity
Phylogenetic relationship
