摘要
目的:观察黄慈颗粒对5-氟尿嘧啶(5-FU)诱导的小鼠认知功能障碍的作用及其机制。方法:用5-FU干预小鼠诱导认知功能障碍模型,随机将小鼠分为对照组、模型组(5-FU,60 mg/kg)、黄慈颗粒低剂量组(1.8 g/kg)和高剂量组(5.4 g/kg)。利用旷场实验和新旧事物识别实验观察各组小鼠运动能力及记忆能力的变化;利用免疫组化技术考察小鼠神经再生及胶质细胞活化情况。结果:①各组小鼠运动能力未见明显差异;②与对照组相比,模型小鼠的记忆能力显著下降(P<0.05),而黄慈颗粒能够显著改善小鼠记忆能力的受损(P<0.05);③与对照组相比,模型组小鼠海马齿状回颗粒下层和侧脑室室管膜下层双皮质素(DCX)的表达显著下降(P<0.05),而低、高剂量黄慈颗粒均可显著提高DCX的表达(P<0.05);④与对照组相比,模型组小鼠海马区小胶质细胞及星形胶质细胞显著增生及活化(P<0.05),而黄慈颗粒能够显著减少海马区小胶质细胞和星型胶质细胞的增殖及激活(P<0.05)。结论:黄慈颗粒可通过改善神经再生受损及胶质增生缓解5-FU诱导的小鼠认知障碍。
Objective:To observe effect and mechanism of Huangci Granules on 5-fluorouracil(5-FU)induced cognitive impairment in mice.Methods:The mice were randomly divided into control group,model group(5-FU,60 mg/kg),low-dose Huangci Granules group(1.8 g/kg)and high-dose Huangci Granules group(5.4 g/kg).The changes of motor ability and memory ability were observed by open field test and new and old objects recognition test.The neurogenesis and gliosis in the brain of mice were investigated by immunohistochemistry in each group.Results:①There was no significant difference in the motor ability of mice in each group.②Compared to the control group,the memory ability of the model mice decreased significantly(P<0.05),and Huangci Granules could significantly improve the impairment of memory ability in mice(P<0.05).③Compared to the control group,the expression of Doublecortin(DCX)in the subgranule zone of the dentate gyrus and subventricular zone of the lateral ventricle decreased significantly in mice of the model group(P<0.05),and both low dose and high dose Huangci Granules could significantly increase the expression of DCX(P<0.05).④Compared to the control group,microglia and astrocytes in the hippocampus of the model group significantly proliferated and activated(P<0.05),and Huangci Granules could significantly reduce the proliferation and activation of microglia and astrocytes in the hippocampus(P<0.05).Conclusion:Huangci Granules can alleviate 5-FU induced cognitive impairment in mice through improving nerve regeneration injury and gliosis.
作者
王杰
贾茹
马福祺
荣汶青
李静茹
冯琴
WANG Jie;JIA Ru;MA Fuqi;RONG Wenqing;LI Jingru;FENG Qing(Department of Oncology,Cancer Institute,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Liver Diseases,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China)
出处
《上海中医药大学学报》
CAS
2022年第S01期124-129,共6页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海中医药大学附属曙光医院四明青年基金(SGKJ-202101)
关键词
黄慈颗粒
5-氟尿嘧啶
神经再生
胶质增生
化疗相关认知障碍
Huangci Granules
5-fluorouracil
nerve regeneration
gliosis
chemotherapy-related cognitive impairment
