摘要
目的探讨狼疮性肾炎(LN)患者血清微小RNA-21(miR-21)与微小RNA-145(miR-145)表达水平及其与疾病活动度的相关性。方法选取宜昌市第二人民医院收治的LN患者120例为LN组,根据SLE疾病活动指数(SLEDAI)将LN患者分为轻度组、中度组、重度组,根据慢性肾脏病分期法将LN患者分为早期、中期、晚期肾损害,同时选取同期体检的70名健康志愿者为对照组。采用实时定量聚合酶链反应(qRT-PCR)法检测血清miR-21、miR-145表达量。检测LN患者临床指标:红细胞沉降率(ESR)、血肌酐(SCr)、24 h尿蛋白定量(UTP)、抗ds-DNA抗体滴度、补体C3、肾小球滤过率(eGFR)、活动性指数(AI)、慢性指数(CI)、肾小管间质病变(TIL)。采用Pearson法分析LN患者血清miR-21、miR-145水平与临床指标的相关性。结果LN组血清miR-21表达水平显著高于对照组(P<0.05),且重度组显著高于轻度组、中度组(P<0.05);LN组血清miR-145表达水平显著降低(P<0.05),且重度组显著低于轻度组、中度组(P<0.05);重度组SCr、ESR、抗ds-DNA抗体、UTP(g/24h)、SLEDAI评分、AI评分、CI评分、TIL评分均显著高于轻度组、中度组(P<0.05),而eGFR、补体C3水平显著降低(P<0.05)。Pearson分析显示重度活动期LN患者ESR、抗ds-DNA抗体、UTP、SLEDAI评分、CI评分、TIL评分均与miR-21呈正相关(P<0.05),而均与miR-145呈负相关(P<0.05)。晚期肾损害LN患者血清miR-21表达水平显著高于早期、中期患者(P<0.05),而血清miR-145表达水平显著降低(P<0.05)。结论LN患者血清miR-21呈高表达,而miR-145呈低表达,二者均与LN疾病活动度及肾损害发生有关,均可作为监测LN疾病活动的血清学指标。
Objective To investigate the expression levels of serum micro RNA-21(miR-21)and micro RNA-145(miR-145)in patients with lupus nephritis(LN),and their correlations with disease activity.Methods 120 cases of LN patients in our hospital were selected as LN group,according to the SLE disease activity index(SLEDAI),the LN patients were divided into mild group,moderate group,and severe group,according to the staging of chronic kidney disease;the LN patients were divided into early stage,middle stage,and advanced renal damage;at the same time,70 healthy volunteers taken physical examination in our hospital were selected as the control group.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the expressions of serum miR-21 and miR-145.The clinical parameters of LN patients were detected:erythrocyte sedimentation rate(ESR),serum creatinine(SCr),24 h urinary protein(UTP),the titer of anti ds-DNA antibody,complement C3,glomerular filtration rate(eGFR),activity index(AI),chronic index(CI),tubulointerstitial lesions(TIL).Pearson method was used to analyze the correlations between serum miR-21 and miR-145 levels with clinical indicators in LN patients.Results The expression level of serum miR-21 in LN group was significantly higher than that in the control group(P<0.05),and level of serum miR-21 in severe disease activity group was significantly higher than that of the mild group and severe group(P<0.05).The expression level of serum miR-145 was lower than that in the control group(P<0.05),and level of serum miR-145 of severe disease activity group was significantly lower than that in mild group and severe group(P<0.05);SCr,ESR,anti ds-DNA antibody,UTP(g/24 h),SLEDAI score,AI score,CI score,and TIL score of LN patients in severe group were significantly higher than those in mild group and moderate group(P<0.05),while the levels of EGFR and complement C3 were significantly decreased(P<0.05);the Pearson analysis showed that ESR,anti-ds-DNA antibody,UTP,SLEDAI score,CI score and TIL score were positively correlated with miR-21 in patients with severe active LN(P<0.05),and were negatively correlated with miR-145(P<0.05);the expression level of serum miR-21 in LN patients with advanced renal damage was significantly higher than that in patients with early stage and middle stage(P<0.05),but the serum miR-145 expression level was significantly decreased(P<0.05).Conclusion In patients with LN,serum miR-21 was highly expressed,while miR-145 was lowly expressed.Both of them were associated with LN disease activity and renal damage,and could be used as serological indicators for monitoring LN disease activity.
作者
雷莉
胡大军
柳永兵
LEI Li;HU Da jun;LIU Yong bing(Department of Nephrology,Second People′s Hospital of Yichang City(Department of Nephrology,Second People′s Hospital of Three Gorges University,Institute of Nephropathy of Integrated Traditional Chinese and Western Medicine,Three Gorges University),Yichang,Hubei 443000,China)
出处
《热带医学杂志》
CAS
2019年第12期1489-1493,共5页
Journal of Tropical Medicine
基金
湖北省自然科学基金(2015CFC813).
