摘要
目的探讨神经调节蛋白4(Nrg4)对2型糖尿病大鼠心肌细胞凋亡的影响。方法将SD大鼠分为对照组(Vehicle)、糖尿病组(DM)和Nrg4干预糖尿病组(Nrg4)。实验结束后,取各组大鼠心肌细胞,流式细胞术检测心肌细胞凋亡率,qRT-PCR检测Bax及Bcl-2的转录表达,Western blot检测凋亡相关蛋白Bax、Bcl-2及Cleaved-caspase 3的表达。结果与Vehicle组相比,DM组心肌细胞凋亡率明显升高[(35.49±1.37)%vs.(11.98±0.85)%;P<0.05],促凋亡蛋白Bax与凋亡执行蛋白Cleaved-caspase 3表达明显增多,抗凋亡蛋白Bcl-2表达明显减少,差异有统计学意义(P<0.05);而Nrg4干预显著抑制糖尿病大鼠心肌细胞凋亡[(19.28±0.96)%vs.(35.49±1.37)%;P<0.05],降低Bax与Cleaved-caspase 3的表达并促进Bcl-2表达(P<0.05)。结论Nrg4干预能缓解2型糖尿病大鼠心肌细胞凋亡。
Objective To investigate the effects of neuregulin 4(Nrg4)on myocardial apoptosis of type 2 diabetic rats.Methods SD rats were randomly divided into vehicle group,diabetes mellites(DM)group and Nrg4 administration group(Nrg4).The apoptosis rate of myocardial cells was detected by flow cytometry,the expression of Bax and Bcl-2 transcription were detected by qRT-PCR,and the expression of apoptosis-related proteins Bax,Bcl-2 and Cleaved caspase 3 were detected by Western blot.Results Compared with the vehicle group,DM significantly promoted cell apoptosis[(35.49±1.37)%vs.(11.98±0.85)%;P<0.05],heightened Bax and cleaved-caspase 3 expression,and reduced Bcl-2 expression(P<0.05).While CBD obviously inhibited cell apoptosis[(19.28±0.96)%vs.(35.49±1.37)%;P<0.05],reduced Bax and cleaved-caspase 3 expression,and increased Bcl-2 expression(P<0.05).Conclusions Nrg4 attenuates myocardial apoptosis of type 2 diabetic rats.
作者
苏静华
吴方丽
焦婷
韩宁
苏静克
张海松
SU Jinghua;WU Fangli;JIAO Ting;HAN Ning;SU Jingke;ZHANG Haisong(Health Medical Center,the Third Medical Center of Chinese PLA General Hospital,Beijing 100039,China;Department of Stomatology,Rocket Force Characteristics Medical Center,Beijing 100088,China;Oncology Division,Affiliated Hospital of Hebei University,Baoding 071000,China;Department of Nephrology,the Third Medical Center of Chinese PLA General Hospital,Beijing 100039,China)
出处
《武警医学》
CAS
2023年第7期557-561,共5页
Medical Journal of the Chinese People's Armed Police Force
