摘要
采用了牛肉中头孢噻呋残留检测的高效液相色谱方法。样品中残留的头孢噻呋,用磷酸缓冲液提取,净化、浓缩后进行HPLC分析。头孢噻呋在0.05~5.0μg/m L的质量浓度范围内呈良好的线性关系;在牛肉组织中添加量分别为200、500、1000μg/kg,添加回收率在85.0%~102%,批内、批间RSD均≤10%。本方法定量准确,结果重复性好,适用于牛肉中头孢噻呋残留定量分析。
A HPLC method was adopted for the determination of ceftiofur residues in beef.Ceftiofur residues in the sample was extracted with phosphate buffer, purified,concentrated and analyzed by HPLC. Ceftiofur residues showed a good linear relationship in the concentration range of 0.05-5.0 μg/m Lthe, addition amount of ceftiofur in beef tissue was 200μg/kg, 500μg/kg and 1000μg/kg, the recovery was 85.0%-102%, and the RSD of intra-assay and inter-assay was less than or equal to 5%. The method is accurate and reproducible. It is suitable for the quantitative analysis of cefotaxif residue in beef.
作者
郭林云
苏亚
李雅宁
于海东
苏日娜
李超
Guo Linyun;Su Ya;Yu Haidong;Li Yaning;Li Chao;Bi Lige(Inner Mongolia Veterinary Drugs Inspection Institute,Hohhot 010020,China)
出处
《内蒙古石油化工》
CAS
2020年第6期29-30,37,共3页
Inner Mongolia Petrochemical Industry
