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贝氏莫尼茨绦虫不同染色方法的比较试验

Comparison of Different Staining Methods for Moniezia benedeni
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摘要 为了制作染色效果良好、构造明晰的贝氏莫尼茨绦虫永久玻片标本,本试验采用孔雀石绿、1%Janus绿、硼砂卡红、苏木素染色方法染色和不染色对贝氏莫尼茨绦虫成熟节片进行处理。结果表明:用苏木精和硼砂胭脂红染色后,虫体结构清晰、分界明显、效果好;用孔雀石绿染色后,染色明显、结构清晰、染色迅速;1%Janus绿染色后,标本透明度低、颜色深浅不清晰、部分虫体构造不清晰、不便于观察。建议在染色教学中使用苏木精和硼砂胭脂红,其次是孔雀石绿。 In order to make the M.benedeni permanent seal sheet specimens with clear staining effect and structure,five staining methods,i.e.,Malachite Green staining,Janus green staining,Borax Carmine staining,Hematoxylin staining,and no staining were used to compare the staining effects of M.benedeni in this paper.The results showed that the mature segments of M.benedeni were stained with the Hematoxylin and Carmine,the structure was clear,the color was layers,and the three-dimensional sense was strong.After staining with the Malachite Green,the color was obvious,the structure was clear,and the dyeing was rapid.After staining with the 1%Janus green,the specimen had the characteristics with low transparency,uneven staining,and unclear structure of some organs,which was not conducive to observation.It was recommended to use the Hematoxylin and borax carmine in dyeing teaching,followed by the Malachite Green.
作者 陆静 阿丽雅 吴敬泽 王瑞 高珍珍 LU Jing;Aliya;WU Jingze;WANG Rui;GAO Zhenzhen(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处 《内蒙古农业大学学报(自然科学版)》 CAS 2022年第4期1-6,共6页 Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金 内蒙古农业大学校级教学改革项目
关键词 莫尼茨绦虫 染色 苏木精 硼砂胭脂红 M.benedeni Dye Hematoxylin Borax carmine
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