摘要
目的:评价传统中草药香加皮的主要活性成分杠柳毒苷致心律失常的风险。方法:采用离体组织(豚鼠乳突肌和兔浦肯野纤维),给予浓度为0、0.3、1.0、3.0μmol/L杠柳毒苷,检测其对动作电位的影响。指标包括:静息电位(resting membrane potential,RMP)、动作电位幅度(action potential amplitude,APA)、最大除极速度(maximal depolarization rate of phase 0,dv/dtmax)、复极30%、50%和90%动作电位时程(action potential duration at 30%,50%and 90%repolarization,APD30、APD50、APD90)。结果:与0μmol/L组比较,0.3和1.0μmol/L杠柳毒苷可缩短豚鼠乳突肌的APD30和APD50;0.3、1.0、3.0μmol/L杠柳毒苷降低兔浦肯野纤维的dv/dtmax;3.0μmol/L杠柳毒苷减少兔浦肯野纤维的APA;1.0、3.0μmol/L杠柳毒苷缩短兔浦肯野纤维的APD30。差异均有统计学意义。结论:杠柳毒苷有潜在诱导心律失常的风险。
Objective:The aim of the study was to evaluate the risk of arrhythmia induced by periplocin,the main active component of Cortex Periplocae,a traditional Chinese medicine preparation.Methods:In this experiment,isolated tissues(guinea pig papillary muscle and rabbit Purkinje fiber)were treated by 0.3,1.0,or 3.0μmol/L periplocin to assess the effect on action potential.The following parameters and endpoints were assessed:resting membrane potential(RMP),action potential amplitude(APA),maximal depolarization rate of phase 0(dv/dtmax),action potential duration at 30%,50%,and 90%repolarization(APD30,APD50,and APD90).Results:As compared to the 0μmol/L group,periplocin-related significant reduction in APD30,APD50 was observed at guinea pig papillary muscle in the 0.3 or 1.0μmol/L periplocin.Periplocin-related significant decrease in dv/dtmaxwas observed at rabbit Purkinje fiber in the 0.3,1.0,or 3.0μmol/L periplocin.Periplocin-related significant reduction in APA was observed at rabbit Purkinje fiber in the 3.0μmol/L periplocin.Periplocin-related significant reduction in APD30 was observed at rabbit Purkinje fiber in the 1.0,or 3.0μmol/L periplocin.Conclusion:Periplocin has the potential to induce arrhythmia.
作者
李旻
顾晓峰
汪溪洁
李华
马璟
LI Min;GU Xiaofeng;WANG Xijie;LI Hua;MA Jing(China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research,Shanghai 201203,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第10期1437-1441,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家重大科技专项(2018ZX09201017-008)
上海市科委实验动物领域项目(17140900700)
