摘要
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination.[Methods]The L9(34)orthogonal test was carried out,the comprehensive scores of ginsenoside Rb1 content,notoginsenoside R1 content,tanshinone I content and saikosaponin A content in the compound extract were used as evaluation indicators to explore the effects of water addition,the water addition,decoction time and decoction times on the water extraction process,and verification tests were carried out.[Results]The optimized water extraction process was as follows:adding 9 times of water in the first extraction,and extracting for 90 min;adding 7 times of water in the second extraction,and extracting for 60 min;adding 7 times of water in the third extraction,and extracting for 60 min.[Conclusions]The optimized water extraction process is stable and feasible,and can be used for the extraction of various herbs in Compound Man Gan Ning.It can also provide experimental basis for the formulation development of Compound Man Gan Ning sustained release tablet.
[Objectives] To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination. [Methods] The L9（34） orthogonal test was carried out, the comprehensive scores of ginsenoside Rb1 content, notoginsenoside R1 content, tanshinone I content and saikosaponin A content in the compound extract were used as evaluation indicators to explore the effects of water addition, the water addition, decoction time and decoction times on the water extraction process, and verification tests were carried out. [Results] The optimized water extraction process was as follows: adding 9 times of water in the first extraction, and extracting for 90 min; adding 7 times of water in the second extraction, and extracting for 60 min; adding 7 times of water in the third extraction, and extracting for 60 min. [Conclusions] The optimized water extraction process is stable and feasible, and can be used for the extraction of various herbs in Compound Man Gan Ning. It can also provide experimental basis for the formulation development of Compound Man Gan Ning sustained release tablet.
基金
Supported by Project of National Natural Science Foundation of China(81360524)
Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0341)
National Traditional Chinese Medicine Characteristic Technology Heritage Talent Training Program(20184828005)
Youth Foundation Project of Guangxi University of Chinese Medicine(2019QN036)
Program of Scientific Research Third-level Laboratory"Chinese(Zhuang)Medicine Chemical and Quality Analysis Laboratory"of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 2009[21]).
