摘要
目的研究祛瘀生新方对脾虚湿蕴型溃疡性结肠炎(ulcerative colitis,UC)患者粪便病毒多样性的影响。方法在不同分类水平比较UC患者与正常人群粪便中病毒多样性的差异,并进行丰度聚类热图分析及相关性分析。结果UC患者粪便样本中有尾噬菌体的丰度明显上升。目水平分类上有尾噬菌体的丰度高于其他类型病毒;科水平分类时,有尾噬菌体中的长尾噬菌体、肌尾噬菌体、短尾噬菌体及一些无名有尾噬菌体丰度也是明显高于其他类型病毒;而在属、种水平分类的表现与目、科水平一致。进一步丰度聚类热图分析结果提示中药治疗能明显降低有尾噬菌体的相对丰度。结论有尾噬菌体与UC的发病密切相关,祛瘀生新方可能通过降低有尾噬菌体的相对丰度达到抑制肠道炎症的治疗目的。
Objective This study aimed to investigate the influence on the fecal virus diversity by Quyu Shengxin Formula(祛瘀生新方,QYSX)in UC patients with spleen deficiency and dampness.Methods The differences of fecal virus diversity between UC group and normal group in different classification levels were studied,then the abundance cluster Heatmap analysis and correlation analysis were carried out.Results The abundance of Caudovirales in the fecal samples of the UC group was significantly increased,which showed that the abundance of Caudovirales was higher than that of other viruses at the order level.The abundance of Caudovirales,Myoviridae,Podoviridae and some unnamed Caudovirales was also significantly higher than that of other viruses at the family level.This characteristic was also manifested in the genus and the species level.The analysis of the abundance clustering Heatmap showed TCM can obviously decrease the relative abundance of Caudovirales.Conclusion Caudovirales are closely related to the onset of UC and QYSX may suppress intestinal inflammation by reducing the relative abundance of phages.
作者
李盈
杨豪杰
干丹
崔灿
金磊
秦凯健
王振宜
吴炯
LI Ying;YANG Haojie;GAN Dan;CUI Can;JIN Lei;QIN Kaijian;WANG Zhenyi;WU Jiong(Yueyang Hospital of Integrative Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《辽宁中医杂志》
CAS
2022年第2期86-89,221-222,共6页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81973859)
国家自然科学基金(青年)项目(81603633)
上海市卫生健康委员会中医药科研项目(2020JQ006)
上海中医药大学附属岳阳中西医结合医院青年孵育项目(2019YYQ26)
关键词
溃疡性结肠炎
肠道微生物
病毒
祛瘀生新
ulcerative colitis
enteric microorganism
virus
eliminating blood stasis and generating new tissues
