摘要
目的研究评价益肾骨康方对骨转移癌痛大鼠的止痛和骨保护作用。方法48只SD大鼠随机分为对照组、模型组、解毒化瘀组、扶正组、益肾骨康方低剂量组、益肾骨康方高剂量组,每组8只。除对照组外其余各组采用大鼠乳腺癌MRMT-1细胞悬液10μL缓慢注射于大鼠胫骨骨髓腔内建立骨转移癌模型。于造模后第2天开始给药,中药组给予相应中药1 mL/100 g灌胃,假手术组及模型组给予等量生理盐水灌胃,每日1次,连续2周。观察各组大鼠机械性痛阈值、热痛阈值变化,X线摄片观察骨质破坏情况,骨密度扫描仪测定各组大鼠胫骨骨密度。结果与模型组比较,解毒化瘀组和益肾骨康方低剂量组在给药第14天提高了骨转移癌痛大鼠机械性痛阈值(P<0.05);扶正组、益肾骨康方低剂量组和益肾骨康方高剂量组较模型组提高了骨转移癌痛大鼠骨密度(P<0.05)。造模后第14天,除对照组外各组大鼠胫骨周围软组织肿胀,单侧骨皮质破坏,髓腔内密度不均,其中模型组大鼠胫骨局部破坏较其余组更加明显,呈虫蚀状。结论益肾骨康方可改善骨转移癌疼痛,减轻骨转移癌导致的溶骨性故破坏。
Objective To study the analgesic and bone protective effects of Yishen Gukang Formula(益肾骨康方)on rats with bone metastatic pain.Methods Forty-eight SD rats were randomly divided into control group,model group,Jiedu Huayu For⁃mula group,Fuzheng group,Yishen Gukang Formula low-dose group and Yishen Gukangfang high-dose group,8 rats in each group.In addition to the control group,the MRMT-1 cell lines were injected into the tibial bone marrow cavity of the rats to es⁃tablish the model of bone metastasis pain.The Chinese medicine groups were given 1 mL/100 g of the corresponding Chinese medicine,while the control group and the model group were given the same amount of normal saline,once a day for 2 weeks con⁃secutively.The changes of mechanical pain thresholds and thermal pain thresholds in each group were observed,the bone destruc⁃tion was observed by X-ray film,and the bone density of the tibia of each group was measured by bone density scanner.Results Compared with those of the model group,the mechanical pain thresholds of the rats with bone metastatic pain was increased in the Jiedu Huayu group and the Yishen Gukang Formula low-dose group on the 14th day(P<0.05).The bone mineral density of rats in the Fuzheng group,Yishen Gukang Formula low and high dose groups were increased compared with those of the model group(P<0.05).On the 14th day,except for the control group,the rats in each group had soft tissue swelling around the tibia,unilater⁃al cortical destruction,and heterogeneous density in the medullary cavity.The local destruction of the tibia in the model group was more obvious than that in the other groups.Conclusion Yishen Gukang Formula can improve the pain of metastatic bone cancer and reduce the osteolytic destruction caused by metastatic bone cancer.
作者
何理玉
梁敏
芦殿荣
冯利
HE Liyu;LIANG Min;LU Dianrong;FENG Li(Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijin 100102,China;National Cancer Cencer/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100021,China)
出处
《辽宁中医杂志》
CAS
2021年第12期187-190,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年项目(81603598)
关键词
乳腺癌骨转移
癌痛
中药
机械性痛觉
热刺激痛觉
breast cancer bone metastasis
cancer pain
traditional Chinese medicine
mechanical pain
heat stimulated pain
