摘要
目的探讨参松养心胶囊对心肌细胞胰岛素样生长因子1(IGF-1)、chemerin表达的影响。方法采用SD仔鼠分离心肌细胞,分为3组,正常组进行常规培养,I/R组通过缺氧复氧方法建立了心肌缺血再灌注损伤模型,胶囊组在模型建立前加入320μmol/L参松养心胶囊溶液预处理24 h。记录心肌细胞IGF-1、chemerin在各时间点的表达情况。结果细胞处理24 h、36 h与48 h后,与正常组比较,I/R组和胶囊组的细胞存活率显著降低(P<0.05),活性氧(ROS)水平显著增加(P<0.05),胶囊组与I/R组比较差异也有统计学意义(P<0.05)。细胞处理48 h后,与正常组比较,I/R组与胶囊组G0/G1期细胞比例增加(P<0.05),IGF-1、chemerin蛋白相对表达量显著增加(P<0.05),S期与G2期/M期细胞比例减少(P<0.05),胶囊组与I/R组比较差异也有统计学意义(P<0.05)。结论参松养心胶囊可抑制心肌细胞IGF-1、chemerin表达,降低细胞ROS水平与平衡细胞周期,提高心肌细胞存活率,发挥对心肌缺血再灌注损伤的保护作用。
Aim To investigate the effects of Shensong Yangxin Capsule on the expression of IGF-1 and chemerin in myocardial cells.Methods Myocardial cells were isolated from SD rats and were divided into three groups.The normal group were routinely cultured,the I/R group were treated by hypoxia-reoxygenation to establish model of myocardial ischemia-reperfusion injury,the Capsule group were added final concentration of 320μmol/L Shensong Yangxin Capsule pretreated for 24 hours before the model were established,and the expression of IGF-1 and chemerin in cardiomyocytes were recorded in different time.Results After 24 h,36 h and 48 h treatment,the cell survival rate of the I/R group and the Capsule group were significantly lower(P<0.05)and the ROS level were significantly increased(P<0.05),the difference between the Capsule group and the I/R group were also statistically significant(P<0.05).After 48 h treatment,compared with the normal group,the ratio of G0/G1 phase cells in the I/R group and the Capsule group were increased(P<0.05),and the relative expression of IGF-1 and chemerin protein were increased significantly(P<0.05).The proportion of cells in the S phase and G2/M phase were decreased(P<0.05),and the difference compared between Capsule group and I/R group were also statistically significant(P<0.05).Conclusion Shensong Yangxin Capsule can inhibit the expression of IGF-1 and chemerin in cardiomyocytes,thereby improving the survival rate of cardiomyocytes,reducing the level of ROS and balancing the cell cycle,and exerting protective effects on myocardial ischemiareperfusion injury.
作者
肖金平
付景秋
李小雷
纪道兵
陈俊
XIAO Jinping;FU Jingqiu;LI Xiaolei;JI Daobing;CHEN Jun(Department of Cardiovascular Medicine,Dongfeng Hospital,Hubei University of Medicine,Shiyan,Hubei 442008,China)
出处
《中国动脉硬化杂志》
CAS
2020年第1期20-24,共5页
Chinese Journal of Arteriosclerosis
基金
湖北省科技计划项目(2016CFB529).
