摘要
目的探讨人脐带间充质干细胞(hUC-MSCs)对人胰腺癌细胞侵袭能力的调控作用及机制。方法使用含10%胎牛血清的DMEM/F12培养基处理胰腺癌细胞MIA-PaCa-2,并将其设为对照组;使用含10%胎牛血清的人脐带间充质干细胞培养基(hUC-MSC-CM)处理胰腺癌细胞MIA-PaCa-2,并将其设为研究组。Transwell实验评估细胞侵袭力,Western blot实验检测核因子κB(NF-κB)及磷酸化NF-κB(p-NF-κB)水平,分析hUC-MSCs调控胰腺癌细胞侵袭能力的机制。结果Transwell结果显示,对照组MIA-PaCa-2细胞侵袭数为(401±12)个,低于研究组的(715±36)个,差异有统计学意义(P<0.05)。Western blot结果显示,与对照组比较,研究组的NF-κB无明显变化(P>0.05);与对照组比较,研究组的p-NF-κB明显下调(P<0.05)。结论hUC-MSCs可能通过激活NF-κB提高了胰腺癌细胞的侵袭能力。
Objective To investigate the role and mechanism of human umbilical cord-mesenchymal stem cells(hUC-MSCs)in invasive of human pancreatic cancer cells.Methods Pancreatic cancer cells MIA-PaCa-2 were treated with DMEM/F12 medium containing 10%fetal bovine serum and set as the control group.Pancreatic cancer cells MIA-PaCa-2 were treated with human umbilical cord-mesenchymal stem cell medium(hUC-MSC-CM)containing 10%fetal bovine serum and set as the experimental group.The invasive ability were detected by Transwell assay.The levels of NF-κB and phosphorylated NF-κB(p-NF-κB)were detected by Western blot,and the mechanism by which hUC-MSCs regulate the invasive ability of pancreatic cancer cells was analyzed.Results The results of Transwell showed that the invasion number of MIA-PaCa-2 cells in the control group was(401±12),which was lower than that in the experimental group(715±36),and the difference was statistically significant(P<0.05).Western blot showed that compared with the control group,the NF-κB of the experimental group had no significant change(P>0.05).Compared with the control group,p-NF-κB in the experimental group was significantly down-regulated(P<0.05).Conclusion hUC-MSCs may improve the invasive ability of pancreatic cancer cells by activating NF-κB.
作者
常旭东
李宏宇
陈江
王煜晔
高聪
刘小毓
韩杰
孙广晨
王诗雨
高飞
CHANG Xu-dong;LI Hong-yu;CHEN Jiang;WANG Yu-ye;GAO Cong;LIU Xiao-yu;HAN Jie;SUN Guang-chen;WANG Shi-yu;GAO Fei(Postgraduate Training Base of General Hospital of Northern Theater Command of Jinzhou Medical University,Shenyang 110016,China;Department of Gastroenterology,General Hospital of Northern Theater Command,Shenyang 110016,China;Dalian Medical University,Dalian 116000,China;China Medical University,Shenyang 110000,China)
出处
《临床军医杂志》
CAS
2022年第12期1211-1214,共4页
Clinical Journal of Medical Officers
基金
辽宁省科学技术计划项目(2019-ZD-1068)
关键词
人脐带间充质干细胞
胰腺癌
核因子ΚB
Human umbilical cord-derived mesenchymal stem cells
Pancreatic cancer
Nuclear factor kappa-B
