摘要
目的观察白藜芦醇(RES)对冲击波致小鼠脑组织炎症损伤的影响,并探讨其可能的分子机制。方法将30只健康雄性C57BL/6小鼠随机分入对照组(Control组)、爆震组(Blast组)、爆震+给药组(RES组),每组各10只。伤后24 h进行Y迷宫试验;采集脑组织标本,HE染色检测脑组织爆震后炎症情况;酶联免疫吸附试验(ELISA)检测血清内炎症因子白细胞介素(IL)-6和IL-1β的表达水平;Western blot检测炎症相关蛋白S100β、肿瘤坏死因子(TNF)-α和信号通路相关蛋白高迁移率族蛋白B(HMGB1)、toll样受体(TLR)4、核因子(NF)-κB的表达。结果与Control组比较,Blast组进入食物臂的次数和位于食物臂的总时间显著减少(P<0.05);与Blast组比较,RES组在食物臂的进入次数有所增加,在食物臂的总时间明显延长(P<0.05)。与Control组比较,Blast组小鼠血清内炎症因子IL-1β和IL-6明显升高(P<0.05);与Blast组比较,RES组小鼠血清内炎症因子IL-1β和IL-6表达降低(P<0.05)。与Control组比较,Blast组小鼠脑组织内存在细胞变性和炎症细胞浸润;与Blast组比较,RES组小鼠脑组织内细胞变性明显缓解,炎症细胞浸润明显减轻。与Control组比较,Blast组小鼠脑组织内炎症相关蛋白TNF-α和S100β明显升高(P<0.05);与Blast组比较,RES组小鼠脑组织内炎症相关蛋白TNF-α和S100β明显降低(P<0.05)。与Control组比较,Blast组小鼠脑组织内HMGB1、TLR4、NF-κB蛋白表达均显著升高(P<0.05);与Blast组比较,RES组小鼠脑组织内HMGB1、TLR4、NF-κB蛋白表达显著减少(P<0.05)。结论RES干预对冲击波致小鼠脑组织炎症损伤有保护作用,其机制可能与抑制HMGB1/TLR4/NF-κB信号通路相关。
Objective To observe the effects of resveratrol(RES)on brain inflammation induced by shock wave in mice,and to explore its possible molecular mechanism.Methods Thirty healthy male C57 BL/6 mice were randomly divided into Control group(Control group),Blast group(Blast group)and Blast+administration group(RES group),with 10 mice in each group.Y maze test was performed 24 hours after injury.Brain tissue samples were collected and HE staining was used to detect the inflammation of brain tissue after explosion.The expression levels of interleukin(IL)-6 and IL-1βin serum were detected by enzyme linked immunosorbent assay(ELISA).Western blot was used to detect the expression of inflammatory related protein S100β,tumor necrosis factor(TNF)-α,high mobility group box 1(HMGB1),toll-like receptors(TLR)4 and nuclear factor(NF)-κB.Results Compared with the Control group,the times of entering the food arm and the total time in the food arm in Blast group were significantly reduced(P<0.05).Compared with Blast group,the number of entries in the food arm was increased in RES group,and the total time in the food arm was significantly prolonged(P<0.05).Compared with Control group,serum levels of inflammatory cytokines IL-1βand IL-6 in Blast group were significantly increased(P<0.05).Compared with Blast group,the expressions of IL-1βand IL-6 in serum of RES group were decreased(P<0.05).Compared with the Control group,there were cell degeneration and inflammatory cell infiltration in the brain tissues of the Blast group.Compared with Blast group,cell degeneration and inflammatory cell infiltration in RES group were significantly alleviated.Compared with Control group,TNF-αand S100βin Blast group were significantly increased(P<0.05).Compared with Blast group,inflammatory related proteins TNF-αand S100βin RES group were significantly decreased(P<0.05).Compared with Control group,the expression of HMGB1,TLR4 and NF-κB protein in Blast group was significantly increased(P<0.05).Compared with Blast group,the expression of HMGB1,TLR4 and NF-κB protein in RES group was significantly decreased(P<0.05).Conclusion The protective effect of RES on brain inflammation induced by shock wave may be related to the inhibition of HMGB1/TLR4/NF-κB signaling pathway.
作者
丛培芳
刘颖
史秀云
马瑞珩
金红旭
CONG Pei-fang;LIU Ying;SHI Xiu-yun;MA Rui-heng;JIN Hong-xu(Department of Emergency Medicine,General Hospital of Northern Theater Command,Shenyang 110016,China)
出处
《临床军医杂志》
CAS
2022年第3期229-232,236,共5页
Clinical Journal of Medical Officers
基金
解放军总后勤部卫生部科研基金(CLB20C036)
2021年度军事训练防治(21XLS21)
辽宁省重点研发计划(2019010205,2021JH2/10300024)
