摘要
为了研究“长白新喹”抗癌药对ANAE阳性淋巴细胞的影响,本文采用酸性α-醋酸萘酯酶(ANAE)反应标记淋巴细胞的细胞化学方法对100例正常瑞士小白鼠尾血的淋巴细胞进行了ANAE阳性百分率测定和分型的工作。结果表明:ANAE阳性者为T淋巴细胞,阴性者为B淋巴细胞。阳性率平均值为62.5±110%。随机观察其中50例,其ANAE阳性率为63.5±0.1%,其中点状颗粒型的淋巴细胞百分率为55.5±0.9%(3粒以下者25.3±0.8%;4-8粒者24.6±1%;9粒以上者4.6±0.3%),而弥散型为8.5±0.3%。在研究“长白新喹”对小白鼠外周血ANAE阳性淋巴细胞影响时,实验组与对照组均接种S_(180)腹水癌细胞。于接种后第3天,实验组用“长白新喹”0.8 mg/0.2 ml腹腔注射,连续7天。两组均有实验前后作为自身对照,实验结果表明:在两组动物之间以及实验前后的自身对照,血液内ANAE阳性淋巴细胞及弥散型淋巴细胞均明显增多(P<0.05)。根据上述结果,本文讨论了抗癌药“长白新喹”对细胞免疫的影响。
The influence of Chang-Bai Xin Kui on the acid alphanaphthy acetate esterase(ANAE)positive lymphocytes was studied by using ANAE as a cytochemical marker.S_(180) cells of ascites tumor were inoculated into the mice in experimental and control group.3 days later,0.8 mg/0.2 ml.of Chang-Bai Xin Kui was injected peritoneally everyday in 7 days.Each of the two groups has its own self control.
作者
陈诗芸
谢锦玉
Cheng Shi-Yun;Xie Jing-Yu(Department of Histology and Embryology,Kunming Medical College;Cytologlcal Dept.of Basic Reaserch Institute,The Academy of Traditional Chinese Medicine of China)
出处
《解剖学杂志》
CAS
1987年第1期24-27,共4页
Chinese Journal of Anatomy
