摘要
本实验采用PAP免疫细胞化学技术,观察P物质和亮-脑啡肽样免疫反应神经元在鼠中脑中缝核的分布并作了计数。1.含P物质样免疫反应神经元胞体仅见于中缝背核最尾侧部,含P物质阳性胞体数平均167个/只;而亮-脑啡肽样免疫反应胞体几乎遍于中缝背核全长,其阳性胞体数平均132个/只。2.亮-脑啡肽样免疫反应胞体主要定位于中缝背核中线区;而P物质样免疫反应胞体除分布于中缝背核中线区以外,也定位在其两侧的延伸。3.亮-脑啡肽样免疫反应胞体恒定地出现在中缝正中核内,平均199个/只;而P物质样免疫反应胞体仅见于2例动物。4.中缝背核内有些含P物质样免疫反应阳性胞体或突起,紧邻血管壁排列,未观察到亮-脑啡肽样免疫反应神经元有类似现象。本实验观察到鼠中缝正中核内存在含P物质样免疫反应神经元胞体。
The different distributions of substance P(SP)-and leucine-enkephalin(L-ENK)-like immunoreactive neurons in the midbrain raphe nuclei of the rats have been studied,using PAP immunocytochemical technique.The results showed four main points:1.SP-containing immunoreactive cell bodies were only seen in the most caudal part of the dorsal raphe nucleus,the average number of SP-positive cell bodies was about 167 in each animal.The L-ENK-like immunoreactive positive cell bodies were found almost in the whole length of the dorsal raphe nucleus,the L-ENK-positive cell bodies were about 132 in each animal.2.L-ENK-Iike immunoreactive perikarya were mainly located in the midline area of the dorsal raphe nucleus.Besides the above areas,SP-containing immunoreactive cell bodies were also located bilaterally in the extended area from the raphe.3.L-ENK-containing immunoreactive cell bodies constantly appeared in the median raphe nuclei,the average number was about 199 in each animal,but SP-containing immunoreactive bodies were only observed in this nucleus of two rats.4.Some SP-like immunoreactive cell bodies or processes were observed in the vincinty of vascular walls,but the L-ENK positive neurons were not found similar results.The present experiment showed that the SP-like immunoreactive perikarya is located in the median raphe nuclei of the rat.
作者
周兰仙
胡家荦
章祖陈
王亚威
Zhou Lanxian;Hu Jialuo;Zhang Zuchen;Wang Yawei(Department of Anatomy,Hubei Medical College,Wuhan)
出处
《解剖学报》
CAS
1988年第3期256-260,340,共6页
Acta Anatomica Sinica
关键词
P物质
亮-脑啡肽
中缝背核
中缝正中核
免疫细胞化学
Substance P
Leucine-Enkephalin
Dorsal raphe nucleus
Median raphe nucleus
Immunocytochemistry
