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实验性小鼠心肌SDH和LDH组织化学等的观察 被引量:2

THE STUDIES OF HISTOCHEMISTRY AND ELECTRON-MICROSCOPIC CYTOCHEMISTRY ON SDH AND LDH IN THE MYOCARDIUM OF EXPERIMENTAL ANOXIC MICE
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摘要 本文报道用光镜和电镜方法研究实验性缺氧小鼠心肌,其琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)的活性和定位均有一定变化,表现为肌原纤维的甲(?)沉淀不均匀,呈断续着色;在电镜下见线粒体膜和嵴,由于酶活性增强而致密度加深。注射10%红参水浸液6天后,缺氧的小鼠心肌损伤则有明显缓和,SDH和LDH话性和定位接近于正常对照组。 With the methods of light microscopic histochemistry and electron-microscopic cytochemistry,we studied the changes of lactate dehydrogenase(LDH)and succinic dehydrogenase(SDH)in the anoxic myocardium of the mice;to which normal saline(INSAM group)or 10%aqueous extract of Ginsengradix rubra(IGRAM group)was injected before the anoxic experiment.The results were as follows:Ⅰ.The Light Microscopic Study:1.In the myocardial sections of the INSAM group,the reaction of SDH of myofibrillae are variegated and the fibrillae vary in thickness.Granular mitochondria are clearly stained and arranged in alignment in the intermyofibrillar spaces.The intercalated discs fail to be stained and their spaces widen.In the IGRAM group,the reactions of SDH are similar to that of the normal control.The enzyme reaction product in the myofibrillae appear as homogeneous diffuse blue precipitate of formazan,the cross striations of myofibrillae are clear and strings of mitochondria are visible.2.The reactions of LDH in the INSAM group reveals that myofibrillar staining is also lost in a length of myofibrilla,strings of mitochondria are clearly stained.The reaction in the IGRAM group is similar to that of the normal control.In the myofibrillae there is a homogenous continuous violet precipitate of formazan.Ⅱ.The Electron-Microscopic Study:1.The reaction of SDH in the INSAM group shows that the enzyme activity in the mitochondrial inner membrane and the cristae is stronger than that of the normal control.The increased activity is represented by the increase of electronic density or darkening and thickening of the membranes.However,the activity in the IGRAM group is simillar to that of the normal control but weaker in reaction.2.The reaction of LDH in the INSAM group reveals that the enzyme activity exhibited by membrane darkening is obviously stronger than that of the normal control but in the IGRAM group it shows clearly a weaker reaction.The results mentioned above suggest that Ginsengradix rubra might alleviate the metabolic disturbance caused by myocardial anoxia.
作者 施联蓉 张一 许祖钵 Shi Lianrong;Zhang Yi;Xu Zubo(Department of Cytology,Institute of Basic Medicine,Beijing Medical College,Beijing;Department of Medicine,the Third Affiliated Hospital,Beijing Medical College,Beijing)
出处 《解剖学报》 CAS 1984年第2期210-215,239,共7页 Acta Anatomica Sinica
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  • 1魏毅,曹庆淑,庄鼎,韩振京.电针对家兔缺血心肌单相动作电位的影响[J]针刺研究,1986(02).
  • 2杨凤乡,薛大海,贾果峰.高原大白鼠心肌SDH的组织化学观察[J]青海医学院学报,1986(02).

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