摘要
本实验用电子显微镜观察了大鼠口饲棉酚后睾丸生精上皮中支持细胞的超微结构的改变。棉酚每日剂量30毫克/公斤体重,连服4~6周后分别杀死取材,制成超薄切片观察。结果表明服棉酚后支持细胞呈现一系列细胞功能活跃与吞噬功能有关的胞器超微结构改变:溶酶体及脂滴增多,线粒体增生和多形性变化,服药6周后,内质网扩张明显,脂滴堆积,细胞开始处于不活跃状态。结合上述结果,本文对服棉酚后支持细胞超微结构变化的性质及其意义进行了讨论。
The present study was designed to investigate the ultrastructural changes of Sertoli cells of rat testis following gossypol administration at a dosage of 30 mg/kg/day for 4~6 weeks.Results indicate that 4 weeks after gossypol treatment,a series of ultrastructural changes concerning with phagocytic activity such as increased in amount of lysosomes,lipid droplets,ring or cup shaped mitochondria as well as the multiform changes of mitochondria and lysosomes were evident in the Sertoli cell.However,by 6 weeks after treatment,the Sertoli cells became"inactive"and began to show degenerative changes:distension and vesiculization of endoplasmic reticulum,accumulation of lipid droplets,cellular debris and lysosomes in varying sizes and phases,and the occurrence of atrophic changes of mitochondria.The nature of these changes in Sertoli cells following gossypol treatment and its significance was discussed.
作者
王代学
游美梅
薛社普
Wang Daixue;You Meimei;Shieh Sher-pu(Department of Cell Biology,Institute of Basic Medical Research,Chinese Academy of Medical Sciences)
出处
《解剖学报》
CAS
1982年第2期211-214,234,共5页
Acta Anatomica Sinica
