摘要
使用农药防治人参病菌会使人参农药残留超标,影响人参药用价值,而使用生防菌防治人参病害是较有潜力的生物防治措施之一。采用土壤稀释法从人参根际土壤中分离得到1株人参锈腐病的生防细菌C10,经鉴定,此菌为解淀粉芽孢杆菌Bacillus amyloliquefaciens。试验结果表明:其抑菌率可达80.95%。在对人参茎块和根部的回接试验中发现,解淀粉芽孢杆菌C10可以显著降低锈腐菌对人参茎块和根部的侵染。从根际微生物多样性分析表明,接种解淀粉芽孢杆菌C10能显著降低人参根际土壤真菌的丰富度指数。该菌的使用对人参锈腐病的防治有积极作用。
The pesticide residues of ginseng will exceed the standard and affect the medicinal value of ginseng.It is one of the potential biological control measures to use biocontrol bacteria to control ginseng diseases.A biocontrol bacterium C10 was isolated from ginseng rhizosphere soil by soil dilution method.It was identified as Bacillus amyloliquefaciens.The results showed that the antibacterial rate of the strain was 80.95%.The results showed that Bacillus amyloliquefaciens C10 could significantly reduce the infection of rust rot fungi on ginseng stems and roots.In addition,microbial diversity analysis showed that inoculation of Bacillus amyloliquefaciens C10 significantly reduced the richness index of fungi in ginseng rhizosphere soil.It can be seen that the use of this fungus has a positive effect on the prevention and control of ginseng rust rot.
作者
张洁婧
陈德国
高继轩
张建峰
田春杰
田磊
ZHANG Jiejing;CHEN Deguo;GAO Jixuan;ZHANG Jianfeng;TIAN Chunjie;TIAN Lei(College of Life Sciences,Jilin Agricultural University,Key Laboratory for Straw Biology and Utilization of the Ministry of Education,Changchun 130118,China;Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2020年第5期526-532,共7页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31370144)
吉林省教育厅项目(JJKH20180687KJ)
关键词
人参
人参锈腐病
生防菌
根际微生物
ginseng
ginseng rust rot
biocontrol strain
rhizosphere microorganism
