摘要
目的:建立肺炎克雷伯菌和耐药基因KPC/CMY-2检测体系。方法:通过设计特异性引物和探针,调节优化荧光定量PCR检测体系的反应条件、反应体系组分用量,同时验证本检测体系灵敏度和特异度。结果:建立的肺炎克雷伯菌和耐药基因KPC/CMY-2检测体系均呈良好的扩增曲线,总耗时90 min。另外,批内、批间、日内、日间精密度验证结果提示两套荧光定量PCR检测体系具有较好的检测灵敏度和精密度。结论:肺炎克雷伯菌和耐药基因KPC/CMY-2检测体系,可为临床提供快速检测碳青霉烯类、三代头孢菌素等抗菌素耐药性提供多重耐药基因分子生物学检测平台,有望对本地区肺炎克雷伯多重耐药基因的流行与感染治疗提供理论与统计学参考依据。
Objective This study aim to establish real-time polymerase chain rection(RT-PCR)detection systems for Klebsiella and resistant genes KPC/CMY-2.Methods To design specific primers and probes and optimize the reaction conditions and components counts,along with sensitivity and specificity verifications for this detection systems.Results The established methods of Klebsiella and resistant genes KPC/CMY-2 have better amplification curves,with reaction time of 90 min.Separately,four different precision verifications of the batch,batch-to-batch,within the day and the daytime have been conducted,confirmed the well sensitivity and specificity with detection system and detection kit.Conclusion This detection systems center for Klebsiella and resistant genes KPC/CMY-2,could immensely contribute to the detection with resistant genes and antibiotics including carbapenem,third generation cephalosprins,which laid theory and statistics foundations for the trends of multi-resistant Klebsiella and clinical treatments.
作者
张毕明
张利
侯正利
谭德勇
孙菁
Zhang Bi-ming;Zhang Li;Hou Zheng-li;Tan De-Yong;Sun Jing(The Fourth Hospital of Changsha,Changsha 410006,China;The Sansure Biotech,Changsha 410006,China)
出处
《湖南师范大学学报（医学版）》
2021年第6期45-48,共4页
Journal of Hunan Normal University(Medical Sciences)
基金
长沙市科技计划项目(kq1907012)
